Nakano T, Ito M, Mizuno T, Mizutani K, Ihara T, Kamiya H, Sakurai M
Department of Pediatrics, Mie University School of Medicine, Japan.
Cell Immunol. 1993 Mar;147(1):73-80. doi: 10.1006/cimm.1993.1049.
To determine the expression of interleukin 2 receptor (IL-2R) and the change of CD45RA and CD45RO antigen on lymphocytes in response to human cytomegalovirus (HCMV), peripheral blood mononuclear cells (PBMC) were cultured with HCMV antigen, and the expression of IL-2R and of CD45RA and CD45RO antigens on lymphocytes was analyzed by flow cytometry. The expression of IL-2R alpha (p55) and -beta (p75) was significantly increased on T cells obtained from CMV-seropositive donors following culture with the CMV antigen. An increased expression of IL-2R alpha and -beta was observed on both CD4+ and CD8+ cells, but the percentage of cells that expressed IL-2R was higher in CD4+ cells. The percentage of CD45RA+ cells was decreased and the percentage of CD45RO+ cells, especially of CD4+CD45RO+ cells, was increased when cultured with the CMV antigen. These observations suggest that switching of CD45RA+ cells to CD45RO+ cells occurred following stimulation with the CMV antigen. The expression of IL-2R and the activation of memory T cells are important to the recovery from and the protection against CMV infection.
为了确定白细胞介素2受体（IL-2R）的表达以及淋巴细胞上CD45RA和CD45RO抗原在人巨细胞病毒（HCMV）刺激后的变化，将外周血单个核细胞（PBMC）与HCMV抗原一起培养，并用流式细胞术分析淋巴细胞上IL-2R以及CD45RA和CD45RO抗原的表达。来自CMV血清阳性供体的T细胞在与CMV抗原培养后，IL-2Rα（p55）和-β（p75）的表达显著增加。在CD4⁺和CD8⁺细胞上均观察到IL-2Rα和-β表达增加，但表达IL-2R的细胞百分比在CD4⁺细胞中更高。当与CMV抗原一起培养时，CD45RA⁺细胞的百分比降低，而CD45RO⁺细胞，尤其是CD4⁺CD45RO⁺细胞的百分比增加。这些观察结果表明，在CMV抗原刺激后发生了CD45RA⁺细胞向CD45RO⁺细胞的转变。IL-2R的表达和记忆性T细胞的激活对于从CMV感染中恢复以及预防CMV感染很重要。
