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利用5'非编码区保守序列进行核酸杂交和核酸扩增检测牛病毒性腹泻病毒的比较

Comparison of nucleic acid hybridization and nucleic acid amplification using conserved sequences from the 5' noncoding region for detection of bovine viral diarrhea virus.

作者信息

Ridpath J F, Bolin S R, Katz J

机构信息

Virology Cattle Research, USDA Agricultural Research Service, Ames, Iowa.

出版信息

J Clin Microbiol. 1993 Apr;31(4):986-9. doi: 10.1128/jcm.31.4.986-989.1993.

DOI:10.1128/jcm.31.4.986-989.1993
PMID:8385157
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC263600/
Abstract

Primers and probes derived from conserved sequences located in the 5' noncoding region of pestiviruses were evaluated for detection of bovine viral diarrhea virus. With these reagents, hybridization and polymerase chain reaction tests detected 62 of 90 and 90 of 90 bovine viral diarrhea virus isolates, respectively. A quick lysis method for preparing RNA for use in polymerase chain reaction amplification also was evaluated.

摘要

对源自瘟病毒5'非编码区保守序列的引物和探针进行了评估，以检测牛病毒性腹泻病毒。使用这些试剂，杂交试验和聚合酶链反应试验分别检测出90株牛病毒性腹泻病毒分离株中的62株和90株。还评估了一种用于制备聚合酶链反应扩增所用RNA的快速裂解方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5500/263600/bcc3ffc2a2d4/jcm00016-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5500/263600/bcc3ffc2a2d4/jcm00016-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5500/263600/bcc3ffc2a2d4/jcm00016-0235-a.jpg

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本文引用的文献

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Viral contamination of bovine fetal lung cultures and bovine fetal serum.牛胎儿肺培养物和牛胎儿血清的病毒污染
Am J Vet Res. 1980 Oct;41(10):1680-1.
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Comparative sequence analysis of the 5' noncoding region of classical swine fever virus strains from Europe, Asia, and America.来自欧洲、亚洲和美洲的经典猪瘟病毒株5'非编码区的比较序列分析。
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Ruminant pestiviruses.反刍动物瘟病毒
Br Vet J. 1995 Nov-Dec;151(6):615-42. doi: 10.1016/s0007-1935(95)80145-6.
8
Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis.利用聚合酶链反应和限制性内切酶分析，从猪、牛和羊中分离出的瘟病毒至少可分为三个基因组。
Arch Virol. 1994;136(3-4):309-23. doi: 10.1007/BF01321060.
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Rapid detection of bovine viral diarrhea virus by using RNA extracted directly from assorted specimens and a one-tube reverse transcription PCR assay.通过使用直接从各种样本中提取的RNA和单管逆转录聚合酶链反应检测法快速检测牛病毒性腹泻病毒。
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