Vilcek S, Herring A J, Herring J A, Nettleton P F, Lowings J P, Paton D J
Moredun Research Institute, Edinburgh, U.K.
Arch Virol. 1994;136(3-4):309-23. doi: 10.1007/BF01321060.
A polymerase chain reaction-based assay capable of detecting a broad range of pestiviruses from pigs, cattle, or sheep was developed. Of six sets of primers selected from different parts of the pestivirus genome, the best results were provided by a pair from the highly conserved 5' non-coding region which gave amplification with all 129 isolates tested. This panel consisted of 33 isolates from pigs, 79 from cattle, and 17 from sheep. Differentiation between the viruses was achieved by cutting the PCR-amplified products with the restriction endonucleases AvaI and Bg1I. Using this procedure it was possible to distinguish at least 3 genogroups; group 1 (HCV) contained 32 of the pig isolates, group II (BVDV) contained all the cattle isolates tested plus 6 sheep isolates and group III (BDV) contained 11 sheep isolates and 1 pig isolate.
开发了一种基于聚合酶链反应的检测方法，该方法能够检测来自猪、牛或羊的多种瘟病毒。从瘟病毒基因组的不同部位选择的六组引物中，来自高度保守的5'非编码区的一对引物给出了最佳结果，对所有129个测试分离株均能进行扩增。该组包括33个猪分离株、79个牛分离株和17个羊分离株。通过用限制性内切酶AvaI和Bg1I切割PCR扩增产物实现病毒之间的区分。使用该程序可以区分至少3个基因群；第1组（HCV）包含32个猪分离株，第II组（BVDV）包含所有测试的牛分离株以及6个羊分离株，第III组（BDV）包含11个羊分离株和1个猪分离株。
