Tsuchida K, Lewis K A, Mathews L S, Vale W W
Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, CA 92037.
Biochem Biophys Res Commun. 1993 Mar 31;191(3):790-5. doi: 10.1006/bbrc.1993.1286.
A cDNA encoding the rat transforming growth factor-beta (TGF-beta) type II receptor was isolated by hybridization from a rat pituitary gland cDNA library. The rat TGF-beta type II receptor comprises 567 amino acid residues with a cysteine-rich extracellular domain, a single transmembrane domain and an intracellular protein kinase domain with predicted serine/threonine specificity. The comparison of the amino acid sequences of the rat and human TGF-beta type II receptors indicated that they are highly conserved particularly in the intracellular kinase domain. RNA blot hybridization and reverse-transcription polymerase chain reaction (RT-PCR) analyses showed that rat TGF-beta type II receptor is widely distributed in various tissues and is expressed abundantly in the ovary and lung.
通过杂交从大鼠垂体cDNA文库中分离出一个编码大鼠转化生长因子β（TGF-β）Ⅱ型受体的cDNA。大鼠TGF-βⅡ型受体由567个氨基酸残基组成，具有富含半胱氨酸的细胞外结构域、单个跨膜结构域和具有预测的丝氨酸/苏氨酸特异性的细胞内蛋白激酶结构域。大鼠和人TGF-βⅡ型受体氨基酸序列的比较表明，它们高度保守，尤其是在细胞内激酶结构域。RNA印迹杂交和逆转录聚合酶链反应（RT-PCR）分析表明，大鼠TGF-βⅡ型受体广泛分布于各种组织中，在卵巢和肺中大量表达。
