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不同生理阶段高邮鸭卵巢中差异表达基因及信号通路的鉴定

Identification of differentially expressed genes and signaling pathways in Gaoyou duck ovary at different physiological stages.

作者信息

Zhang Lei, Xie Jun, Sun Guobo, Ji Rongchao, Li Xiaoming, Zhang Xue, Wang Jian

机构信息

School of Animal Science, Jiangsu Agri-animal Husbandry Vocational College, Taizhou, China.

出版信息

Front Vet Sci. 2023 May 3;10:1190998. doi: 10.3389/fvets.2023.1190998. eCollection 2023.

DOI:10.3389/fvets.2023.1190998
PMID:37206435
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10189055/
Abstract

INTRODUCTION

Gaoyou duck is famous in China and abroad for its good production of double-yolk eggs. However, there has been no systematic research on the egg-laying characteristics of the Gaoyou duck, which limits the development and utilization of breed resource.

METHODS

To identify the essential genes related to ovarian development, the transcriptome profiles of the ovaries of Gaoyou ducks at different physiological stages were analyzed. The transcriptome profiles of the ovaries of Gaoyou ducks at 150 d (before laying), 240 d (egg laying) and 500 d (nesting) were constructed, and the differentially expressed genes (DEGs) underwent GO (gene ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses.

RESULTS

The 6 randomly selected DEGs were verified by real-time fluorescent quantitative PCR that their relative expression was consistent with the transcriptional expression profile. Furthermore, KEGG analysis found that 8 candidate signaling pathways were essential for ovarian development, including the MAPK signaling pathway, Progesterone-mediated oocyte maturation, Cell adhesion molecules (CAMs), NOD-like receptor signaling pathway, ECM-receptor interaction, Focal adhesion, TGF-beta signaling path-way and Phagosome. Finally, 5 key DEGs were identified to participate in ovarian development, including TGIF1, TGFBR2, RAF1, PTK2 and FGF10.

DISCUSSION

Our findings reveal the mechanisms under-lying the molecular regulation of related genes in Gaoyou duck ovarian development.

摘要

引言

高邮鸭以其良好的双黄蛋生产能力而闻名于国内外。然而，目前尚未对高邮鸭的产蛋特性进行系统研究，这限制了该品种资源的开发和利用。

方法

为了鉴定与卵巢发育相关的关键基因，对不同生理阶段的高邮鸭卵巢转录组图谱进行了分析。构建了150日龄（产蛋前）、240日龄（产蛋期）和500日龄（抱窝期）高邮鸭卵巢的转录组图谱，并对差异表达基因（DEGs）进行了基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析。

结果

通过实时荧光定量PCR对随机选取的6个差异表达基因进行验证，其相对表达量与转录表达谱一致。此外，KEGG分析发现8条候选信号通路对卵巢发育至关重要，包括丝裂原活化蛋白激酶（MAPK）信号通路、孕酮介导的卵母细胞成熟、细胞黏附分子（CAMs）、核苷酸结合寡聚化结构域样受体（NOD样受体）信号通路、细胞外基质受体相互作用、粘着斑、转化生长因子-β（TGF-β）信号通路和吞噬体。最后，鉴定出5个参与卵巢发育的关键差异表达基因，包括TGIF1、TGFBR2、RAF1、PTK2和FGF10。

讨论

我们的研究结果揭示了高邮鸭卵巢发育相关基因的分子调控机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/2128671480d7/fvets-10-1190998-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/9bc821ec7505/fvets-10-1190998-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/efff5459ebb6/fvets-10-1190998-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/091671e4e5ec/fvets-10-1190998-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/31ec3fa42a63/fvets-10-1190998-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/e7ea4d4d11c2/fvets-10-1190998-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/dd0d25c26a63/fvets-10-1190998-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/f0ca41d5c216/fvets-10-1190998-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/8ac04ab9391b/fvets-10-1190998-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/2128671480d7/fvets-10-1190998-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/9bc821ec7505/fvets-10-1190998-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/efff5459ebb6/fvets-10-1190998-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/091671e4e5ec/fvets-10-1190998-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/31ec3fa42a63/fvets-10-1190998-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/e7ea4d4d11c2/fvets-10-1190998-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/dd0d25c26a63/fvets-10-1190998-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/f0ca41d5c216/fvets-10-1190998-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/8ac04ab9391b/fvets-10-1190998-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2d/10189055/2128671480d7/fvets-10-1190998-g0009.jpg

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