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酵母果糖1,6-二磷酸酶结构基因的突变影响果糖1,6-二磷酸酶-内切葡聚糖酶A融合蛋白的表达。

Mutations in the yeast fructose 1,6-biphosphatase structural gene affect expression of a fructose 1,6-biphosphatase-endoglucanase A hybrid protein.

作者信息

Silva A, Benitez J

机构信息

Facultad de Biologia, Universidad de La Habana, Cuba.

出版信息

Curr Genet. 1993;23(4):370-2. doi: 10.1007/BF00310902.

DOI:10.1007/BF00310902
PMID:8385582
Abstract

Mutations in the yeast fructose 1,6 biphosphatase structural gene severely reduced expression of a fructose 1,6 biphosphatase-endoglucanase A hybrid protein introduced into yeast on multicopy or centromeric vectors. Upon glucose limitation the mutant yeasts were incapable of de-repressing endoglucanase A synthesis.

摘要

酵母果糖1,6 -二磷酸酶结构基因的突变严重降低了通过多拷贝或着丝粒载体导入酵母中的果糖1,6 -二磷酸酶-内切葡聚糖酶A融合蛋白的表达。在葡萄糖受限的情况下，突变酵母无法解除对内切葡聚糖酶A合成的抑制。

相似文献

1
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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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RECIPROCAL EFFECTS OF CARBON SOURCES ON THE LEVELS OF AN AMP-SENSITIVE FRUCTOSE-1,6-DIPHOSPHATASE AND PHOSPHOFRUCTOKINASE IN YEAST.碳源对酵母中一种AMP敏感型果糖-1,6-二磷酸酶和磷酸果糖激酶水平的相互影响
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Kinetic differences between two interconvertible forms of fructose-1,6-bisphosphatase from Saccharomyces cerevisiae.酿酒酵母中果糖-1,6-二磷酸酶两种可相互转化形式之间的动力学差异。
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Fructose bisphosphatase of Saccharomyces cerevisiae. Cloning, disruption and regulation of the FBP1 structural gene.酿酒酵母的果糖双磷酸酶。FBP1结构基因的克隆、破坏与调控。
J Mol Biol. 1985 Nov 20;186(2):307-19. doi: 10.1016/0022-2836(85)90107-x.
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Regulation of yeast fructose-1,6-bisphosphatase in strains containing multicopy plasmids coding for this enzyme.
FEBS Lett. 1988 Dec 19;242(1):149-52. doi: 10.1016/0014-5793(88)81004-4.
10
Secretion and glycosylation of Clostridium thermocellum endoglucanase A encoded by the celA gene in Saccharomyces cerevisiae.酿酒酵母中由celA基因编码的嗜热栖热放线菌内切葡聚糖酶A的分泌与糖基化
Yeast. 1989 Jul-Aug;5(4):299-306. doi: 10.1002/yea.320050410.