Donadio S, Staver M J
Abbott Laboratories, Abbott Park, IL 60064.
Gene. 1993 Apr 15;126(1):147-51. doi: 10.1016/0378-1119(93)90604-2.
The Saccharopolyspora erythraea eryAI and eryAII genes, which, together with eryAIII, are responsible for the formation of the macrolactone portion of the antibiotic erythromycin, are separated by a 1.46-kb segment, designated IS1136, with the characteristics of an insertion sequence. It contains an open reading frame of 425 codons similar to that of the Anabaena IS891 and is present in four nonidentical copies in the Sac. erythraea genome. Inverted repeats were found near the ends of IS1136, and in the copy in eryA, one of the ends was found to overlap the 5' end of eryAII. Hybridization analysis suggests that IS1136 is confined to Saccharopolyspora species containing eryA-homologous DNA.
糖多孢红霉菌的eryAI和eryAII基因与eryAIII共同负责抗生素红霉素大环内酯部分的形成，它们被一个1.46 kb的片段隔开，该片段命名为IS1136，具有插入序列的特征。它包含一个425个密码子的开放阅读框，与鱼腥藻IS891的开放阅读框相似，并且在糖多孢红霉菌基因组中以四个不同的拷贝存在。在IS1136的末端附近发现了反向重复序列，在eryA中的拷贝中，其中一个末端与eryAII的5'末端重叠。杂交分析表明，IS1136仅限于含有eryA同源DNA的糖多孢菌属物种。
