Gaisser S, Böhm G A, Cortés J, Leadlay P F
University of Cambridge, Department of Biochemistry, UK.
Mol Gen Genet. 1997 Oct;256(3):239-51. doi: 10.1007/s004380050566.
The gene cluster (ery) governing the biosynthesis of the macrolide antibiotic erythromycin A by Saccharopolyspora erythraea contains, in addition to the eryA genes encoding the polyketide synthase, two regions containing genes for later steps in the pathway. The region 5' of eryA, and lying between eryA and the gene eryK, which is known to encode the C-12 hydroxylase, has been sequenced and shown to contain seven additional open reading frames (ORFs 13-19). On the basis of sequence similarities, roles are proposed for several of these ORFs in the biosynthesis of the deoxysugar mycarose and the deoxyaminosugar desosamine. A chromosomal mutant carrying a deletion in ORF15 has been constructed and shown to accumulate 3-O-mycarosylerythronolide B, as expected for an eryC mutant. Similarly, a chromosomal mutant carrying a deletion in ORF16 has been constructed and shown to accumulate erythronolide B, as expected for an eryB mutant.
由糖多孢红霉菌合成大环内酯类抗生素红霉素A的基因簇（ery），除了编码聚酮合酶的eryA基因外，还包含该途径后期步骤相关基因的两个区域。eryA 5'端且位于eryA与已知编码C-12羟化酶的基因eryK之间的区域已测序，结果显示含有另外七个开放阅读框（ORF13 - 19）。基于序列相似性，推测其中几个ORF在脱氧糖鼠李糖和脱氧氨基糖地红霉素的生物合成中发挥作用。构建了一个在ORF15中带有缺失的染色体突变体，结果表明其积累了3 - O - 鼠李糖基红霉素B，正如eryC突变体所预期的那样。同样，构建了一个在ORF16中带有缺失的染色体突变体，结果表明其积累了红霉素内酯B，正如eryB突变体所预期的那样。
