Melkert P W, Hopman E, van den Brule A J, Risse E K, van Diest P J, Bleker O P, Helmerhorst T, Schipper M E, Meijer C J, Walboomers J M
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
Int J Cancer. 1993 Apr 1;53(6):919-23. doi: 10.1002/ijc.2910530609.
The prevalence of human papillomavirus (HPV) genotypes in relation to age was investigated by the polymerase chain reaction (PCR) method in cytologically normal smears from 4 different groups of women. Group A consisted of young women from a district population, aged 15-34 years, using oral contraceptives and visiting general practitioners for a check-up (n = 156); group B were asymptomatic women, aged 35-55, in a district population participating in a triennial screening program for cervical cancer (n = 1555); group C and D consisted of women, seen at the gynecological outpatient department for a wide spectrum of gynecological complaints or for control of their hormonal contraception, aged 15-34 years (n = 2320), and aged 35-55 years (n = 1826) respectively. An HPV (all types) prevalence of 14.1%, 4.1%, 13.9% and 6.6% and an HPV 16/18 prevalence of 3.8%, 0.9%, 3.3% and 1.5% were found in groups A, B, C and D respectively. Statistically significant differences (p value < 0.001) in HPV prevalence were found between women aged 15-34 years and women aged 35-55 years in the district population and in the hospital population. No statistically significant differences in HPV 16/18 were observed after age-matching between women in corresponding age-classes of both populations. In a 5-year interval analysis a strong age-dependent relationship was demonstrated, with a maximum between 20 and 24 years. After the age of 35 a constant level of 1-2% HPV 16/18 was observed. These results indicate that genital HPV infections are age-dependent and suggest that HPV infections at young age can be transient. The implications of these findings in the context of cervical cancer screening are discussed.
采用聚合酶链反应（PCR）方法，对4组不同女性的细胞学正常涂片进行研究，以调查人乳头瘤病毒（HPV）基因型的流行情况与年龄的关系。A组由来自某地区的年轻女性组成，年龄在15至34岁之间，使用口服避孕药并因体检就诊于全科医生（n = 156）；B组是某地区无症状女性，年龄在35至55岁之间，参加每三年一次的宫颈癌筛查项目（n = 1555）；C组和D组分别由因各种妇科疾病或激素避孕检查而就诊于妇科门诊的女性组成，年龄在15至34岁之间（n = 2320）以及35至55岁之间（n = 1826）。在A、B、C和D组中分别发现HPV（所有类型）的流行率为14.1%、4.1%、13.9%和6.6%，HPV 16/18的流行率分别为3.8%、0.9%、3.3%和1.5%。在该地区人群和医院人群中，15至34岁女性与35至55岁女性之间的HPV流行率存在统计学显著差异（p值<0.001）。在对两组人群相应年龄组的女性进行年龄匹配后，未观察到HPV 16/18的统计学显著差异。在一项为期5年的间隔分析中，显示出强烈的年龄依赖性关系，在20至24岁之间达到峰值。35岁以后，观察到HPV 16/18的水平恒定在1 - 2%。这些结果表明，生殖器HPV感染与年龄有关，并提示年轻时的HPV感染可能是短暂的。讨论了这些发现在宫颈癌筛查背景下的意义。
