Chin K, Song S K, Cheong J H, Ko K H, Kuroiwa Y
National Institute of Safety Research, Seoul, Korea.
J Toxicol Sci. 1993 Feb;18(1):21-9. doi: 10.2131/jts.18.21.
Selectivity of lead effect on dopamine beta-hydroxylase activity in regions of brai nfrom rats postnatally exposed to lead was tested. Three groups of animals were prepared; (1) Rats exposed to lead at a low dose (0.05% PbAcetate: PbAc); (2) Rats exposed to lead at a high dose (0.2% PbAc); (3) Age-matched normal control rats. At 2, 4, 6 and 8 weeks of age weight of whole brain and body in each group were measured. At the same ages activities of dopamine beta-hydroxylase and Na+K(+)-ATPase were measured in 5 brain regions of each animal. Exposure of rats to lead generally decreased Na+/K(+)-ATPase activity and showed alternative changes of dopamine beta-hydroxylase activity were detected without concomitant changes of Na+/K(+)-ATPase activity were telencephalon and pons/medulla at 2 weeks of age and telencephalon, diencephalon and pons/medulla at 4 weeks of age and midbrain and pons/medulla at 6 weeks of age and cerebellum at 8 weeks of age in rats exposed to lead at a low dose, and those in rats exposed to lead at a high dose were midbrain at 6 weeks of age and cerebellum at 8 weeks of age. These data imply that noradrenergic nervous system in the brain regions described above could selectively be affected by lead.
测试了出生后暴露于铅的大鼠大脑区域中铅对多巴胺β-羟化酶活性的影响的选择性。制备了三组动物：（1）低剂量暴露于铅的大鼠（0.05%醋酸铅：PbAc）；（2）高剂量暴露于铅的大鼠（0.2%PbAc）；（3）年龄匹配的正常对照大鼠。在2、4、6和8周龄时，测量每组动物的全脑和体重。在相同年龄，测量每只动物5个脑区中多巴胺β-羟化酶和Na+K(+)-ATP酶的活性。大鼠暴露于铅通常会降低Na+/K(+)-ATP酶活性，并且在2周龄时端脑和脑桥/延髓、4周龄时端脑、间脑和脑桥/延髓、6周龄时中脑和脑桥/延髓以及8周龄时小脑，检测到多巴胺β-羟化酶活性的交替变化，且Na+/K(+)-ATP酶活性无伴随变化，低剂量铅暴露大鼠如此，高剂量铅暴露大鼠在6周龄时为中脑，8周龄时为小脑。这些数据表明，上述脑区中的去甲肾上腺素能神经系统可能会被铅选择性地影响。
