Elgendy H M, Norman M E, Keaton A R, Laurencin C T
Harvard-MIT, Cambridge 02139.
Biomaterials. 1993;14(4):263-9. doi: 10.1016/0142-9612(93)90116-j.
An osteogenic cell line (MC3T3-E1) was used to study the potential of bioerodible polymers and ceramics to support osteoblast growth for a proposed bone-polymer composite for skeletal tissue repair. MC3T3-E1 cells were seeded on to 50:50 poly(lactide-co-glycolide), hydroxyapatite, 50:50 hydroxyapatite/poly(lactide-co-glycolide), and the poly(anhydride), poly(bis(p-carboxyphenoxy) propane surfaces. Cell attachment and growth on these surfaces was found to be highest on poly(lactide-co-glycolide), the least on hydroxyapatite and hydroxyapatite/poly(lactide-co-glycolide) combinations gave intermediate values. The order of adhesion and growth of MC3T3-E1 cells on the polymer and ceramic systems was poly(lactide-co-glycolide) is greater than hydroxyapatite/poly(lactide-co-glycolide) which is greater than hydroxyapatite. Negligible growth was found on poly(bis(p-carboxyphenoxy) propane. High alkaline phosphatase activity for the cells grown on poly(lactide-co-glycolide) and hydroxyapatite/poly(lactide-co-glycolide) confirmed retention of the osteoblast phenotype. This in vitro evaluation suggests that poly(lactide-co-glycolide) and hydroxyapatite/poly(lactide-co-glycolide) combinations may be candidate biomaterials for the construction of a cell-polymer matrix for skeletal tissue regeneration.
一种成骨细胞系（MC3T3-E1）被用于研究可生物降解聚合物和陶瓷对于支持成骨细胞生长的潜力，该研究针对一种用于骨骼组织修复的骨-聚合物复合材料。MC3T3-E1细胞被接种到50:50聚（丙交酯-共-乙交酯）、羟基磷灰石、50:50羟基磷灰石/聚（丙交酯-共-乙交酯）以及聚酸酐、聚（双（对羧基苯氧基）丙烷表面上。结果发现，细胞在这些表面上的附着和生长在聚（丙交酯-共-乙交酯）上最高，在羟基磷灰石上最少，而羟基磷灰石/聚（丙交酯-共-乙交酯）组合的值处于中间。MC3T3-E1细胞在聚合物和陶瓷体系上的黏附及生长顺序为：聚（丙交酯-共-乙交酯）大于羟基磷灰石/聚（丙交酯-共-乙交酯）大于羟基磷灰石。在聚（双（对羧基苯氧基）丙烷上发现细胞生长可忽略不计。在聚（丙交酯-共-乙交酯）和羟基磷灰石/聚（丙交酯-共-乙交酯）上生长的细胞具有高碱性磷酸酶活性，证实其保留了成骨细胞表型。这种体外评估表明，聚（丙交酯-共-乙交酯）和羟基磷灰石/聚（丙交酯-共-乙交酯）组合可能是用于构建骨骼组织再生的细胞-聚合物基质的候选生物材料。
