Yao Jun, Radin Shula, Reilly Gwendolen, Leboy Phoebe S, Ducheyne Paul
Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
J Biomed Mater Res A. 2005 Dec 15;75(4):794-801. doi: 10.1002/jbm.a.30494.
A previous study demonstrated that the incorporation of bioactive glass (BG) into poly (lactic-co-glycolic acid) (PLGA) can promote the osteoblastic differentiation of marrow stromal cells (MSCs) on PLGA by promoting the formation of a calcium-phosphate-rich layer on its surface. To further understand the mechanisms underlying the osteogenic effect of PLGA-BG composite scaffolds, whether solution-mediated factors derived from composite scaffolds/hybrids can promote osteogenesis of marrow stromal cells was tested. The dissolution product from PLGA-30%BG scaffold stimulated osteogenesis of MSCs, as was confirmed by increased mRNA expression of osteoblastic markers such as osteocalcin (OCN), alkaline phosphatase (ALP), and bone sialoprotein (BSP). The three-dimensional structure of the scaffolds may contribute to the production of cell-derived factors that promoted distant MSC differentiation. Thus PLGA-BG composites demonstrate significant potential as a bone-replacement material.
先前的一项研究表明，将生物活性玻璃（BG）掺入聚乳酸-乙醇酸共聚物（PLGA）中，可以通过促进其表面富含磷酸钙层的形成，来促进骨髓基质细胞（MSC）在PLGA上的成骨分化。为了进一步了解PLGA-BG复合支架成骨作用的潜在机制，测试了复合支架/混合物中溶液介导的因子是否能促进骨髓基质细胞的成骨作用。PLGA-30%BG支架的溶解产物刺激了MSC的成骨作用，这一点通过成骨标志物如骨钙素（OCN）、碱性磷酸酶（ALP）和骨唾液蛋白（BSP）的mRNA表达增加得到证实。支架的三维结构可能有助于产生促进远处MSC分化的细胞衍生因子。因此，PLGA-BG复合材料作为骨替代材料具有巨大的潜力。
