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用于高可变基因组PCR扩增的引物设计。

Design of primers for PCR amplification of highly variable genomes.

作者信息

Dopazo J, Rodríguez A, Sáiz J C, Sobrino F

机构信息

INIA-Sanidad Animal, Madrid, Spain.

出版信息

Comput Appl Biosci. 1993 Apr;9(2):123-5. doi: 10.1093/bioinformatics/9.2.123.

DOI:10.1093/bioinformatics/9.2.123
PMID:8386978
Abstract

A program to aid in the search of primers for specific polymerase chain reaction (PCR) amplification of highly variable genomes is presented. It involves the derivation of variability profiles to identify optimal regions for PCR amplification, taking into account stability of DNA-primer hybrids. An application of the program to foot-and-mouth disease virus diagnosis is presented.

摘要

本文介绍了一个用于辅助搜索引物的程序，该引物用于对高度可变基因组进行特异性聚合酶链反应（PCR）扩增。它涉及推导变异性图谱，以确定PCR扩增的最佳区域，同时考虑DNA-引物杂交体的稳定性。本文还介绍了该程序在口蹄疫病毒诊断中的应用。

相似文献

1
Design of primers for PCR amplification of highly variable genomes.用于高可变基因组PCR扩增的引物设计。
Comput Appl Biosci. 1993 Apr;9(2):123-5. doi: 10.1093/bioinformatics/9.2.123.
2
A computer program for the design of PCR primers for diagnosis of highly variable genomes.用于设计诊断高变基因组的PCR引物的计算机程序。
J Virol Methods. 1993 Feb;41(2):157-65. doi: 10.1016/0166-0934(93)90123-9.
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Primer design for specific diagnosis by PCR of highly variable RNA viruses: typing of foot-and-mouth disease virus.
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Evaluation of primers for PCR amplification of RNA polymerase gene sequences of foot-and-mouth disease virus.口蹄疫病毒RNA聚合酶基因序列PCR扩增引物的评估
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A RT-PCR assay for the differential diagnosis of vesicular viral diseases of swine.一种用于猪水泡性病毒病鉴别诊断的逆转录聚合酶链反应检测方法。
J Virol Methods. 1998 Jun;72(2):227-35. doi: 10.1016/s0166-0934(98)00032-9.
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Detection of foot-and-mouth disease viral sequences in clinical specimens and ethyleneimine-inactivated preparations by the polymerase chain reaction.
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Detection of foot-and-mouth disease virus in nasal swabs of asymptomatic cattle by RT-PCR within 24 hours.
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8
Rapid and sensitive polymerase chain reaction based detection and typing of foot-and-mouth disease virus in clinical samples and cell culture isolates, combined with a simultaneous differentiation with other genomically and/or symptomatically related viruses.基于聚合酶链反应的快速灵敏检测及口蹄疫病毒在临床样本和细胞培养分离物中的分型，同时与其他基因组和/或症状相关病毒进行鉴别。
Arch Virol. 1996;141(2):331-44. doi: 10.1007/BF01718403.
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Detection of foot-and-mouth disease virus RNA in clinical samples and cell culture isolates by amplification of the capsid coding region.通过衣壳编码区扩增检测临床样本和细胞培养分离物中的口蹄疫病毒RNA
J Virol Methods. 1993 Apr;42(1):53-61. doi: 10.1016/0166-0934(93)90176-r.
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Rapid and sensitive detection of foot-and-mouth disease virus in tissues by enzymatic RNA amplification of the polymerase gene.
J Virol Methods. 1991 Sep-Oct;34(2):161-72. doi: 10.1016/0166-0934(91)90096-i.

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