用于高可变基因组PCR扩增的引物设计。
Design of primers for PCR amplification of highly variable genomes.
作者信息
Dopazo J, Rodríguez A, Sáiz J C, Sobrino F
机构信息
INIA-Sanidad Animal, Madrid, Spain.
出版信息
Comput Appl Biosci. 1993 Apr;9(2):123-5. doi: 10.1093/bioinformatics/9.2.123.
Abstract
A program to aid in the search of primers for specific polymerase chain reaction (PCR) amplification of highly variable genomes is presented. It involves the derivation of variability profiles to identify optimal regions for PCR amplification, taking into account stability of DNA-primer hybrids. An application of the program to foot-and-mouth disease virus diagnosis is presented.
摘要
本文介绍了一个用于辅助搜索引物的程序，该引物用于对高度可变基因组进行特异性聚合酶链反应（PCR）扩增。它涉及推导变异性图谱，以确定PCR扩增的最佳区域，同时考虑DNA-引物杂交体的稳定性。本文还介绍了该程序在口蹄疫病毒诊断中的应用。
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