Dopazo J, Sobrino F
INIA-Sanidad Animal, Madrid, Spain.
J Virol Methods. 1993 Feb;41(2):157-65. doi: 10.1016/0166-0934(93)90123-9.
PCRDiag (Diagnosis by PCR) is a computer program which allows the localization of pairs of oligonucleotides with optimal thermodynamic requirements for use in a PCR assay. The program is designed for the selection of pairs of primers complementary to sequences present in a group, whose identification is intended, but are absent in other non-specific sequences. The program constitutes a powerful tool, specially in systems which display a high degree of sequence heterogeneity, as is the case of RNA viruses. The program runs on IBM-PC and compatible computers and has no special software requirements. It does not need the previous alignment of the sequences analyzed.
PCRDiag（通过聚合酶链式反应进行诊断）是一个计算机程序，它能够定位具有最佳热力学条件的寡核苷酸对，以用于聚合酶链式反应检测。该程序旨在选择与目标组中存在的序列互补但在其他非特异性序列中不存在的引物对。该程序是一个强大的工具，特别是在像RNA病毒那样具有高度序列异质性的系统中。该程序可在IBM-PC及其兼容计算机上运行，且没有特殊的软件要求。它不需要对所分析的序列进行预先比对。
