Nussbaum O, Laster J, Loyter A
Department of Biological Chemistry, Hebrew University of Jerusalem, Israel.
Exp Cell Res. 1993 May;206(1):11-5. doi: 10.1006/excr.1993.1114.
Incubation of fluorescently labeled Sendai, influenza, as well as Semliki Forest viruses with bull sperm cells resulted in fluorescence dequenching. Fluorescence dequenching was observed with Sendai virus at pH 7.4 while with influenza and Semliki Forest viruses at pH 5.0, a pH value which is required for triggering their fusogenic activity. Control experiments performed with nonfusogenic Sendai and influenza viruses, or with bull sperm cells from which the viral receptors have been removed by treatment with neuraminidase, showed little fluorescence dequenching. These results clearly indicate that animal enveloped viruses are able to interact and to fuse with bull sperm cells. The possibility that following virus-sperm fusion spermatozoa can serve as a carrier of the virus genome and introduce it into recipient eggs during fertilization is discussed.
用荧光标记的仙台病毒、流感病毒以及塞姆利基森林病毒与公牛精子细胞共同孵育，导致荧光猝灭。在pH 7.4条件下，仙台病毒可观察到荧光猝灭，而在pH 5.0条件下，流感病毒和塞姆利基森林病毒可观察到荧光猝灭，pH 5.0是触发它们融合活性所需的值。用非融合性仙台病毒和流感病毒，或用经神经氨酸酶处理去除了病毒受体的公牛精子细胞进行的对照实验，几乎没有显示荧光猝灭。这些结果清楚地表明，动物包膜病毒能够与公牛精子细胞相互作用并融合。文中讨论了病毒与精子融合后，精子是否可以作为病毒基因组的载体，并在受精过程中将其引入受体卵子的可能性。
