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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Nichols J R, Maldonado H S

Department of Biology, Abilene Christian University, TX 79699-8199.

Gen Pharmacol. 1993 Mar;24(2):349-52. doi: 10.1016/0306-3623(93)90315-o.

The effects of ouabain and dihydroouabain (DHO) on Na(+)-K+ ATPase from frog skin were investigated. 2. Ouabain seemed to be a more effective inhibitor, reaching near maximum inhibition at 10(-5) M while DHO reached near maximum at 10(-3) M. 3. Both inhibitors acted within the first few minutes, but ouabain inhibited more strongly over virtually the whole 2 hr considered. 4. Pre-incubation of the enzyme with ATP or Na+ reduced the level of inhibition due to subsequent ouabain or DHO, but preincubation with K+ did not.

研究了哇巴因和二氢哇巴因（DHO）对蛙皮钠钾ATP酶的影响。2. 哇巴因似乎是一种更有效的抑制剂，在10^(-5) M时达到接近最大抑制，而DHO在10^(-3) M时达到接近最大抑制。3. 两种抑制剂在最初几分钟内起作用，但在整个2小时的观察期内，哇巴因的抑制作用更强。4. 酶与ATP或Na+预孵育可降低随后哇巴因或DHO引起的抑制水平，但与K+预孵育则没有这种效果。

Nichols J R, Maldonado H S

Department of Biology, Abilene Christian University, TX 79699-8199.

Gen Pharmacol. 1993 Mar;24(2):349-52. doi: 10.1016/0306-3623(93)90315-o.

The effects of ouabain and dihydroouabain (DHO) on Na(+)-K+ ATPase from frog skin were investigated. 2. Ouabain seemed to be a more effective inhibitor, reaching near maximum inhibition at 10(-5) M while DHO reached near maximum at 10(-3) M. 3. Both inhibitors acted within the first few minutes, but ouabain inhibited more strongly over virtually the whole 2 hr considered. 4. Pre-incubation of the enzyme with ATP or Na+ reduced the level of inhibition due to subsequent ouabain or DHO, but preincubation with K+ did not.

研究了哇巴因和二氢哇巴因（DHO）对蛙皮钠钾ATP酶的影响。2. 哇巴因似乎是一种更有效的抑制剂，在10^(-5) M时达到接近最大抑制，而DHO在10^(-3) M时达到接近最大抑制。3. 两种抑制剂在最初几分钟内起作用，但在整个2小时的观察期内，哇巴因的抑制作用更强。4. 酶与ATP或Na+预孵育可降低随后哇巴因或DHO引起的抑制水平，但与K+预孵育则没有这种效果。