Bednár B, Mandys V
Hlavův I. patologicko-anatomický ústav 1. LF UK, Praha.
Cesk Patol. 1993 Feb;29(1):3-5.
Cell degradation of various origin often starts with an ubiquitinization of damaged proteins and with later production of roughly fibrillar electron-microscopical inclusions. There is a strong immunohistological positivity of ubiquitin in the early intracellular phase. It tends toward an apoptotic lysis either ending with extracellular immunohistologically inert refuse of amyloid type or representing various inter-steps of the degradation process. The ubiquitinization is the most common in neurons and paraneurons. A general importance of ubiquitinization is proved by ubiquitin fibrillar inclusions which were observed in growing old tissue cultures as an expression of programmed cell death. Immunodetection of increased ubiquitin is labour-saving as compared with some classical methods detecting similar lesions particularly in neuropathology.
各种来源的细胞降解通常始于受损蛋白质的泛素化以及随后大致呈纤维状的电子显微镜包涵体的产生。在细胞内早期阶段，泛素具有很强的免疫组织学阳性。它倾向于凋亡性裂解，要么以淀粉样类型的细胞外免疫组织学惰性残渣结束，要么代表降解过程的各种中间步骤。泛素化在神经元和副神经元中最为常见。泛素纤维状包涵体在衰老的组织培养物中被观察到，作为程序性细胞死亡的一种表现，证明了泛素化的普遍重要性。与一些检测类似病变的经典方法相比，尤其是在神经病理学中，免疫检测增加的泛素更为省力。
