Fukuda T, Kakihara T, Kamishima T, Ohnishi Y, Naito M, Kishi K, Shibata A
Second Department of Pathology, Niigata University School of Medicine, Japan.
Leuk Res. 1993 Apr;17(4):325-32. doi: 10.1016/0145-2126(93)90019-h.
Human myelomonocytic leukemic cell line, designated as KY-821, and its sublines KY-Ra, KY-VCR, and KY-MTX, which were resistant to cytosine arabinoside, vincristine, and methotrexate, respectively, were compared for response to various hematopoietic growth factors. Cells of KY-Ra and KY-VCR proliferated in response to natural interleukin-1 (nIL-1), whereas the proliferation of KY-821 and KY-MTX was inhibited. Unexpectedly, recombinant IL-1 alpha and IL-1 beta had no effect on the proliferation of each cell line. The effect of nIL-1 was partially deleted by an addition of optimal anti-IL-1. Supernatants of each cell line had no IL-1 activity. Interferon gamma (IFN gamma) and tumor necrosis factor alpha (TNF alpha) also had an inhibitory effect for KY-821 and KY-MTX, but lacked such effect in KY-RA and KY-VCR. nIL-1, IFN gamma and TNF alpha could not differentiate between any of the cell lines but IFN gamma and TNF alpha induced monocytic surface antigens. In addition, there was no difference in the number of IL-1 and TNF alpha receptors in each cell line. These results indicate that there is a difference in biological effects between nIL-1 and recombinant IL-1 species and acquirement of resistance for some types of drugs may associate closely with different responses to hematopoietic growth factors, probably through altered postmembranous transduction.
人骨髓单核细胞白血病细胞系，命名为KY - 821，及其亚系KY - Ra、KY - VCR和KY - MTX，它们分别对阿糖胞苷、长春新碱和甲氨蝶呤具有抗性，对各种造血生长因子的反应进行了比较。KY - Ra和KY - VCR细胞对天然白细胞介素 - 1（nIL - 1）有增殖反应，而KY - 821和KY - MTX的增殖受到抑制。出乎意料的是，重组白细胞介素 - 1α和白细胞介素 - 1β对每个细胞系的增殖均无影响。添加最佳抗白细胞介素 - 1可部分消除nIL - 1的作用。每个细胞系的上清液均无白细胞介素 - 1活性。干扰素γ（IFNγ）和肿瘤坏死因子α（TNFα）对KY - 821和KY - MTX也有抑制作用，但对KY - RA和KY - VCR缺乏这种作用。nIL - 1、IFNγ和TNFα无法区分任何细胞系，但IFNγ和TNFα可诱导单核细胞表面抗原。此外，每个细胞系中白细胞介素 - 1和肿瘤坏死因子α受体的数量没有差异。这些结果表明，nIL - 1和重组白细胞介素 - 1种类在生物学效应上存在差异，对某些类型药物的抗性获得可能与对造血生长因子的不同反应密切相关，可能是通过改变膜后转导实现的。
