Yanagisawa K, Watanabe I, Inoue Y, Horiuchi T, Hasegawa H, Yasukawa M, Fujita S
First Department of Internal Medicine, Ehime University, School of Medicine, Japan.
J Interferon Cytokine Res. 1996 Sep;16(9):685-93. doi: 10.1089/jir.1996.16.685.
The effects of tumor necrosis factor-alpha (TNF-alpha) were examined in three subclone cells from human myelomonocytic leukemia cell line ME-1. These three subclone cells exhibit different differentiation stages of the myelomonocytic lineage. TNF-alpha exerted a growth-suppressive effect on the least mature subclone cells, ME-F2 cells. On the other hand, TNF-alpha induced the most mature ME-F1 cells and intermediate ME-F3 cells to differentiate along the monocytic pathway. TNF-alpha also enhanced interferon-gamma (IFN-gamma)-induced complement C2 production by ME-F1 and ME-F3 cells but did not affect production by differentiated ME-F1 and ME-F3 cells. These results suggest that the diversity of the effects of TNF on subclone cells from ME-1 depends on the stage of cell differentiation.
在源自人骨髓单核细胞白血病细胞系ME-1的三个亚克隆细胞中检测了肿瘤坏死因子-α（TNF-α）的作用。这三个亚克隆细胞表现出骨髓单核细胞谱系的不同分化阶段。TNF-α对最不成熟的亚克隆细胞ME-F2细胞具有生长抑制作用。另一方面，TNF-α诱导最成熟的ME-F1细胞和中间的ME-F3细胞沿单核细胞途径分化。TNF-α还增强了干扰素-γ（IFN-γ）诱导的ME-F1和ME-F3细胞补体C2的产生，但不影响分化后的ME-F1和ME-F3细胞的产生。这些结果表明，TNF对ME-1亚克隆细胞作用的多样性取决于细胞分化阶段。
