Litzinger M J, Grover B B, Saderup S, Abbott J R
Department of Pediatrics, University of Utah, Salt Lake City 84142.
Int J Dev Neurosci. 1993 Feb;11(1):17-24. doi: 10.1016/0736-5748(93)90031-8.
Voltage sensitive calcium channel (VSCC) probes 125I-omega-GVIA Conotoxin (omega-GVIA), (+)-[5-methyl-3H]-PN200-110 (3H-PN200), and 3H-Nimodipine were bound to developing Swiss Webster mouse whole brain from postnatal days 3 to 24. 125I-omega-GVIA binding, thought to be presynaptic, showed a 50% increase between days 11 and 14. 3H-dihydropyridine binding, thought to be postsynaptic, showed spike patterns when measured developmentally. 3H-PN200 binding showed a > 150% increase between days 11 and 15. 3H-Nimodipine binding showed a > 100% increase between days 11 and 14. Depolarization-induced 45Ca fluxes also increased between days 8 and 16 by > 500%. The dramatic increases indicated by these binding data correspond to a critical period described by Himwich (Int. Rev. Neurobiol. 4, 117, 1962) between postnatal days 11 and 14 in Swiss Webster mice; during this critical period, dendrites exhibit rapid outgrowth, sensory modalities come on line, EEG patterns mature, and the cortex reaches adult proportions. We conclude from these data that the increase in VSCC activity parallels a critical period in the development of the central nervous system in Swiss Webster mice.
电压敏感性钙通道（VSCC）探针125I-ω-芋螺毒素（ω-GVIA）、（+）-[5-甲基-3H]-PN200-110（3H-PN200）和3H-尼莫地平与出生后第3天至第24天发育中的瑞士韦伯斯特小鼠全脑结合。被认为是突触前的125I-ω-GVIA结合在第11天至第14天之间增加了50%。被认为是突触后的3H-二氢吡啶结合在发育测量时呈现出峰值模式。3H-PN200结合在第11天至第15天之间增加了超过150%。3H-尼莫地平结合在第11天至第14天之间增加了超过100%。去极化诱导的45Ca通量在第8天至第16天之间也增加了超过500%。这些结合数据表明的显著增加与Himwich（《国际神经生物学评论》4, 117, 1962）所描述的瑞士韦伯斯特小鼠出生后第11天至第14天的关键期相对应；在这个关键期内，树突迅速生长，感觉模式开始出现，脑电图模式成熟，并且皮质达到成年比例。我们从这些数据得出结论，VSCC活性的增加与瑞士韦伯斯特小鼠中枢神经系统发育中的一个关键期平行。
