Murphy R A, Oger J, Saide J D, Blanchard M H, Arnason B G, Hogan C, Pantazis N J
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Bacteriophage immunoassays, radioimmunoassays, and biological assays have been used to measure levels of NGF in media conditioned by rat C-6 glioma cells in culture. By all three criteria, these cells secrete a macromolecule which is indistinguishable from mouse submandibular gland NGF.
噬菌体免疫测定法、放射免疫测定法和生物学测定法已被用于测量培养的大鼠C-6胶质瘤细胞所分泌培养基中的神经生长因子(NGF)水平。通过这三种标准方法测定发现,这些细胞分泌的一种大分子与小鼠颌下腺神经生长因子无法区分。