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培养的中枢神经系统胶质瘤细胞分泌神经生长因子。

Secretion of nerve growth factor by central nervous system glioma cells in culture.

作者信息

Murphy R A, Oger J, Saide J D, Blanchard M H, Arnason B G, Hogan C, Pantazis N J

出版信息

J Cell Biol. 1977 Mar;72(3):769-73. doi: 10.1083/jcb.72.3.769.

Abstract

Bacteriophage immunoassays, radioimmunoassays, and biological assays have been used to measure levels of NGF in media conditioned by rat C-6 glioma cells in culture. By all three criteria, these cells secrete a macromolecule which is indistinguishable from mouse submandibular gland NGF.

摘要

噬菌体免疫测定法、放射免疫测定法和生物学测定法已被用于测量培养的大鼠C-6胶质瘤细胞所分泌培养基中的神经生长因子(NGF)水平。通过这三种标准方法测定发现,这些细胞分泌的一种大分子与小鼠颌下腺神经生长因子无法区分。

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