Colombaioni L, Strettoi E
Istituto di Neurofisiologia del CNR, Pisa, Italy.
Vis Neurosci. 1993 May-Jun;10(3):395-402. doi: 10.1017/s0952523800004636.
We have investigated by immunofluorescence the appearance of immunoreactive guanosine 3'-5' cyclic monophosphate phosphodiesterase (cGMP-PDE) during the postnatal development of the retina of the pigmented rat. We show that a sudden increase in immunoreactivity takes place during postnatal day five (P5), when rod outer segments begin to form; immunoreactivity develops rapidly in the following days. Labeling is restricted to the developing photoreceptor outer segments, sparing other retinal cells, as confirmed by electron microscopy immunocytochemistry. In addition, cGMP-PDE immunoreactivity follows a center-to-periphery gradient paralleling photoreceptor differentiation. It appears that cGMP-PDE is expressed when the photoreceptor subcellular compartments are already formed, and represents a specific marker of late photoreceptor differentiation. The appearance of cGMP-PDE during development is temporally correlated with the appearance of other proteins of the phototransduction machinery.
我们通过免疫荧光法研究了色素沉着大鼠视网膜出生后发育过程中免疫反应性鸟苷 3'-5' 环磷酸磷酸二酯酶（cGMP-PDE）的出现情况。我们发现，在出生后第 5 天（P5），当视杆细胞外节开始形成时，免疫反应性突然增加；在接下来的几天里，免疫反应性迅速发展。如电子显微镜免疫细胞化学所证实，标记仅限于发育中的光感受器外节，而其他视网膜细胞则未被标记。此外，cGMP-PDE 免疫反应性呈现出从中心到周边的梯度，与光感受器分化平行。似乎 cGMP-PDE 在光感受器亚细胞区室已经形成时表达，并且代表了晚期光感受器分化的特异性标志物。发育过程中 cGMP-PDE 的出现与光转导机制的其他蛋白质的出现存在时间上的相关性。
