Lipkin V M, Dumler I L, Muradov K G, Artemyev N O, Etingof R N
Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
FEBS Lett. 1988 Jul 18;234(2):287-90. doi: 10.1016/0014-5793(88)80100-5.
Monoclonal antibodies were prepared to the gamma-subunit of the cGMP phosphodiesterase. One of them gamma p-1, suppresses the activation of phosphodiesterase through the alpha-subunit of transducin. The gamma-subunit fragment 24-45 rich in Arg and Lys residues is involved in gamma p-1 binding and is essential for the gamma-subunit interaction with transducin. Carboxypeptidase Y cleaves off seven amino acid residues from the C-terminus of the gamma-subunit resulting in phosphodiesterase activation. Thus, the C-terminal fragment of gamma-subunit participates in phosphodiesterase inhibition.
制备了针对cGMP磷酸二酯酶γ亚基的单克隆抗体。其中一种抗体γ p-1可通过转导蛋白的α亚基抑制磷酸二酯酶的激活。富含精氨酸和赖氨酸残基的γ亚基片段24 - 45参与γ p-1的结合，并且对于γ亚基与转导蛋白的相互作用至关重要。羧肽酶Y从γ亚基的C末端切除七个氨基酸残基，导致磷酸二酯酶激活。因此，γ亚基的C末端片段参与磷酸二酯酶的抑制作用。
