快速色谱法测定尿液和全血中的多胺。
Rapid chromatographic method to determine polyamines in urine and whole blood.
作者信息
Adler H, Margoshes M, Snyder L R, Spitzer C
出版信息
J Chromatogr. 1977 Mar 1;143(2):125-36. doi: 10.1016/s0378-4347(00)81818-6.
A procedure is described for the rapid determination of putrascine, spermine and spermidine in ruine and whole blood. The samples are hydrolyzed with barium hydroxide and are neutralized with sulfuric acid. The polyamines are concentrated and separated from amino acids on a small bed of ion-exchange resin that then serves to load the samples on a two-channel, automated ion-exchange chromatography apparatus. As many as 100 samples can be analyzed in a 24-h period. The method has been shown to be applicable to the analysis of urine and whole blood samples, but further development is needed for application to serum samples.
本文描述了一种快速测定尿液和全血中腐胺、精胺和亚精胺的方法。样品用氢氧化钡水解，并用硫酸中和。多胺在一小床离子交换树脂上浓缩并与氨基酸分离，然后将样品加载到双通道自动离子交换色谱仪上。24小时内可分析多达100个样品。该方法已被证明适用于尿液和全血样品的分析，但应用于血清样品还需要进一步改进。
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