硫代乙酰胺改变胞苷酸转移酶活性的调节。

Thioacetamide alters the regulation of cytidylyltransferase activity.

作者信息

Aylagas H, Osada J, Cascales C, Cascales M, Miró-Obradors M J, Palacios-Alaiz E

机构信息

Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Zaragoza, Spain.

出版信息

Biochem Mol Biol Int. 1993 Feb;29(2):307-15.

PMID:8388293

Abstract

Using the weak hepatocarcinogen thioacetamide, we found a 73% decrease in microsomal CTP: Phosphocholine cytidylyltransferase (CT) activity after two months of treatment. We investigated the effect of different effectors on this enzyme activity. Results show that incubation of liver homogenates of treated animals in the presence of either oleate or spermine restored control values of microsomal activity. Incubation of thioacetamide homogenates in the presence of cAMP showed no changes in CT activity, while incubation of control and thioacetamide homogenates in the presence of Ca2+ induced an activation in microsomal activity, although of less intensity in thioacetamide homogenates than in control preparations. Results suggest that thioacetamide alters the regulation of cytidylyltransferase activity.

摘要

使用弱致癌物硫代乙酰胺，我们发现治疗两个月后微粒体CTP：磷酸胆碱胞苷转移酶（CT）活性降低了73%。我们研究了不同效应物对该酶活性的影响。结果表明，在油酸或精胺存在的情况下孵育经处理动物的肝脏匀浆，可恢复微粒体活性的对照值。在cAMP存在的情况下孵育硫代乙酰胺匀浆，CT活性无变化，而在Ca2+存在的情况下孵育对照和硫代乙酰胺匀浆，可诱导微粒体活性激活，尽管硫代乙酰胺匀浆中的激活强度低于对照制剂。结果表明硫代乙酰胺改变了胞苷转移酶活性的调节。