Pollice A, Ciaramella M, Pulitzer J F
Dipartimento di Genetica e Biologia Generale e Molecolare, Facoltà di Scienze Università di Napoli Federico II, Italy.
FEBS Lett. 1993 May 24;323(1-2):77-82. doi: 10.1016/0014-5793(93)81452-6.
The Polyoma virus enhancer (A + B domain) activates transcription in Saccharomyces cerevisiae when joined to appropriate yeast promoter elements. We demonstrate by DNase I footprints and inhibition of binding by specific antibody, that the yeast protein RAP1 binds to the B-domain of the Polyoma enhancer and, at least in some promoter contexts, is responsible for transcriptional activity of the enhancer B-domain in yeast. Close matches to a consensus RAP1-binding site are also present in other viral enhancers.
多瘤病毒增强子（A + B 结构域）与合适的酵母启动子元件相连时，可在酿酒酵母中激活转录。我们通过 DNase I 足迹实验以及特异性抗体对结合的抑制作用证明，酵母蛋白 RAP1 与多瘤病毒增强子的 B 结构域结合，并且至少在某些启动子环境中，负责增强子 B 结构域在酵母中的转录活性。其他病毒增强子中也存在与 RAP1 结合位点共有序列的紧密匹配。
