Ciaramella M, Sacco M, Pulitzer J F
International Institute of Genetics and Biophysics (CNR), Naples, Italy.
Nucleic Acids Res. 1988 Sep 26;16(18):8847-68. doi: 10.1093/nar/16.18.8847.
We have constructed simple test-plasmids to study transcriptional enhancers in yeast. In this paper the reporter-gene is a plasmid borne deletion-substitution derivative (his-del4) of the Saccharomyces cerevisiae HIS3 gene in which the native promoter has been replaced by a dormant, susceptible pseudopromoter. We investigate the function in yeast of foreign control elements, the polyomavirus enhancer and some of its derivatives, inserted in either orientation at the 3' or 5' ends of the reporter gene. The polyoma enhancer (and, although less thoroughly studied, the SV40 enhancer) will strongly activate transcription from latent start sites within the pseudo-promoter sequence. The rules we draw for the polyoma enhancer effect in yeast are, with a few interesting exceptions, remarkably similar to those discovered by experimentation in mammalian cells.
我们构建了简单的测试质粒来研究酵母中的转录增强子。在本文中，报告基因是酿酒酵母HIS3基因的质粒携带的缺失替代衍生物（his-del4），其中天然启动子已被一个休眠的、敏感的假启动子所取代。我们研究了插入到报告基因3'或5'端的任意方向的外源控制元件、多瘤病毒增强子及其一些衍生物在酵母中的功能。多瘤病毒增强子（以及虽然研究较少但SV40增强子）将强烈激活来自假启动子序列内潜在起始位点的转录。我们得出的关于多瘤病毒增强子在酵母中作用的规则，除了一些有趣的例外，与在哺乳动物细胞中通过实验发现的规则非常相似。
