猿猴病毒40小t抗原结合两个锌离子。
Simian virus 40 small-t antigen binds two zinc ions.
作者信息
Turk B, Porras A, Mumby M C, Rundell K
机构信息
Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois 60611-3008.
出版信息
J Virol. 1993 Jun;67(6):3671-3. doi: 10.1128/JVI.67.6.3671-3673.1993.
Abstract
Six cysteine residues of the simian virus 40 small-t antigen (small-t) are important for stability of the protein. Stability has been shown to be related to the ability of small-t to bind zinc ions in vitro. Purified small-t expressed either in bacteria or from baculovirus vectors binds two molecules of zinc per molecule of protein. Thus, small-t may resemble GAL4, which contains a Zn(II)2Cys6 binuclear cluster.
摘要
猿猴病毒40小t抗原（小t）的六个半胱氨酸残基对该蛋白质的稳定性很重要。稳定性已被证明与小t在体外结合锌离子的能力有关。在细菌中或从杆状病毒载体表达的纯化小t每分子蛋白质结合两个锌分子。因此，小t可能类似于GAL4，后者含有一个Zn(II)2Cys6双核簇。
相似文献
1
Simian virus 40 small-t antigen binds two zinc ions.猿猴病毒40小t抗原结合两个锌离子。
J Virol. 1993 Jun;67(6):3671-3. doi: 10.1128/JVI.67.6.3671-3673.1993.
2
Effect of zinc ions on the biochemical behavior of simian virus 40 small-t antigen expressed in bacteria.锌离子对在细菌中表达的猿猴病毒40小t抗原生化行为的影响。
J Virol. 1992 Mar;66(3):1746-51. doi: 10.1128/JVI.66.3.1746-1751.1992.
3
GAL4 transcription factor is not a "zinc finger" but forms a Zn(II)2Cys6 binuclear cluster.GAL4转录因子不是“锌指”，而是形成一个Zn(II)2Cys6双核簇。
Proc Natl Acad Sci U S A. 1990 Mar;87(6):2077-81. doi: 10.1073/pnas.87.6.2077.
4
The zinc finger region of simian virus 40 large T antigen is needed for hexamer assembly and origin melting.猴病毒40大T抗原的锌指区域是六聚体组装和起始点解链所必需的。
J Virol. 1991 Jun;65(6):3167-74. doi: 10.1128/JVI.65.6.3167-3174.1991.
5
Two conditional tsA mutant simian virus 40 T antigens display marked differences in thermal inactivation.
J Virol. 1992 Nov;66(11):6517-26. doi: 10.1128/JVI.66.11.6517-6526.1992.
6
Differential interaction of temperature-sensitive simian virus 40 T antigens with tumor suppressors pRb and p53.温度敏感型猿猴病毒40 T抗原与肿瘤抑制因子pRb和p53的差异相互作用
J Virol. 1996 Oct;70(10):7224-7. doi: 10.1128/JVI.70.10.7224-7227.1996.
7
Simian virus 40 large T antigen targets the microtubule-stabilizing protein TACC2.猿猴病毒40大T抗原靶向微管稳定蛋白TACC2。
J Cell Sci. 2009 Sep 1;122(Pt 17):3190-8. doi: 10.1242/jcs.049627. Epub 2009 Aug 11.
8
Interaction of simian virus 40 small-T antigen produced in bacteria with 56K and 32K proteins of animal cells.细菌中产生的猿猴病毒40小T抗原与动物细胞的56K和32K蛋白的相互作用。
J Virol. 1985 Oct;56(1):325-7. doi: 10.1128/JVI.56.1.325-327.1985.
9
trans-Dominant and non-trans-dominant mutant simian virus 40 large T antigens show distinct responses to ATP.反式显性和非反式显性突变猿猴病毒40大T抗原对ATP表现出不同反应。
J Virol. 1997 Oct;71(10):7549-59. doi: 10.1128/JVI.71.10.7549-7559.1997.
10
A novel simian virus 40 early-region domain mediates transactivation of the cyclin A promoter by small-t antigen and is required for transformation in small-t antigen-dependent assays.一种新型猿猴病毒40早期区域结构域介导小t抗原对细胞周期蛋白A启动子的反式激活，并且在小t抗原依赖性检测中是转化所必需的。
J Virol. 1996 Oct;70(10):6902-8. doi: 10.1128/JVI.70.10.6902-6908.1996.
引用本文的文献
1
Iron‑sulfur clusters in viral proteins: Exploring their elusive nature, roles and new avenues for targeting infections.病毒蛋白中的铁硫簇：探索其难以捉摸的性质、作用以及靶向感染的新途径。
Biochim Biophys Acta Mol Cell Res. 2024 Jun;1871(5):119723. doi: 10.1016/j.bbamcr.2024.119723. Epub 2024 Apr 8.
2
Functional Domains of the Early Proteins and Experimental and Epidemiological Studies Suggest a Role for the Novel Human Polyomaviruses in Cancer.早期蛋白的功能结构域以及实验和流行病学研究表明新型人类多瘤病毒在癌症中发挥作用。
Front Microbiol. 2022 Feb 18;13:834368. doi: 10.3389/fmicb.2022.834368. eCollection 2022.
3
The Oncogenic Small Tumor Antigen of Merkel Cell Polyomavirus Is an Iron-Sulfur Cluster Protein That Enhances Viral DNA Replication.默克尔细胞多瘤病毒的致癌小肿瘤抗原是一种铁硫簇蛋白，可增强病毒DNA复制。
J Virol. 2015 Nov 25;90(3):1544-56. doi: 10.1128/JVI.02121-15. Print 2016 Feb 1.
4
Role of Zn2+ ions in host-virus interactions.锌离子在宿主-病毒相互作用中的作用。
J Virol. 2008 Dec;82(23):11486-94. doi: 10.1128/JVI.01314-08. Epub 2008 Sep 10.
5
SV40 reporter viruses.SV40报告病毒
J Virol Methods. 2008 Jun;150(1-2):7-13. doi: 10.1016/j.jviromet.2008.02.013. Epub 2008 Apr 9.
6
Small tumor antigen of polyomaviruses: role in viral life cycle and cell transformation.多瘤病毒的小肿瘤抗原：在病毒生命周期和细胞转化中的作用
J Cell Physiol. 2008 May;215(2):309-19. doi: 10.1002/jcp.21326.
7
Structural basis of PP2A inhibition by small t antigen.小t抗原对PP2A抑制作用的结构基础
PLoS Biol. 2007 Aug;5(8):e202. doi: 10.1371/journal.pbio.0050202.
8
Simian virus 40 small t antigen mediates conformation-dependent transfer of protein phosphatase 2A onto the androgen receptor.猿猴病毒40小t抗原介导蛋白磷酸酶2A向雄激素受体的构象依赖性转移。
Mol Cell Biol. 2005 Feb;25(4):1298-308. doi: 10.1128/MCB.25.4.1298-1308.2005.
9
Binding specificity of protein phosphatase 2A core enzyme for regulatory B subunits and T antigens.蛋白磷酸酶2A核心酶对调节性B亚基和T抗原的结合特异性。
J Virol. 1999 Jan;73(1):839-42. doi: 10.1128/JVI.73.1.839-842.1999.
10
The amino-terminal transforming region of simian virus 40 large T and small t antigens functions as a J domain.猴病毒40大T抗原和小t抗原的氨基末端转化区作为一个J结构域发挥作用。
Mol Cell Biol. 1997 Aug;17(8):4761-73. doi: 10.1128/MCB.17.8.4761.
本文引用的文献
1
Properties of simian virus 40 small t antigen overproduced in bacteria.在细菌中过量产生的猿猴病毒40小t抗原的特性。
J Virol. 1981 Feb;37(2):683-97. doi: 10.1128/JVI.37.2.683-697.1981.
2
Monoclonal antibodies specific for simian virus 40 tumor antigens.针对猴病毒40肿瘤抗原的单克隆抗体。
J Virol. 1981 Sep;39(3):861-9. doi: 10.1128/JVI.39.3.861-869.1981.
3
Interaction of simian virus 40 small-T antigen produced in bacteria with 56K and 32K proteins of animal cells.细菌中产生的猿猴病毒40小T抗原与动物细胞的56K和32K蛋白的相互作用。
J Virol. 1985 Oct;56(1):325-7. doi: 10.1128/JVI.56.1.325-327.1985.
4
Purification and functional properties of simian virus 40 large and small T antigens overproduced in insect cells.在昆虫细胞中过量表达的猿猴病毒40大T抗原和小T抗原的纯化及功能特性
J Virol. 1988 Aug;62(8):2951-9. doi: 10.1128/JVI.62.8.2951-2959.1988.
5
Zinc release from Xenopus transcription factor IIIA induced by chemical modifications.化学修饰诱导非洲爪蟾转录因子IIIA释放锌离子。
Biochemistry. 1989 Dec 12;28(25):9790-5. doi: 10.1021/bi00451a037.
6
Calculation of protein extinction coefficients from amino acid sequence data.根据氨基酸序列数据计算蛋白质消光系数
Anal Biochem. 1989 Nov 1;182(2):319-26. doi: 10.1016/0003-2697(89)90602-7.
7
Activation of SV40 DNA replication in vitro by cellular protein phosphatase 2A.细胞蛋白磷酸酶2A在体外激活SV40 DNA复制
EMBO J. 1989 Dec 1;8(12):3891-8. doi: 10.1002/j.1460-2075.1989.tb08568.x.
8
The zinc finger region of simian virus 40 large T antigen.猿猴病毒40大T抗原的锌指结构域
J Virol. 1989 Jan;63(1):94-100. doi: 10.1128/JVI.63.1.94-100.1989.
9
Mutational analysis of simian virus 40 small-t antigen.猿猴病毒40小t抗原的突变分析
J Virol. 1990 Jun;64(6):2895-900. doi: 10.1128/JVI.64.6.2895-2900.1990.
10
Association of protein phosphatase 2A with polyoma virus medium tumor antigen.蛋白磷酸酶2A与多瘤病毒中肿瘤抗原的关联。
Proc Natl Acad Sci U S A. 1990 Apr;87(7):2521-5. doi: 10.1073/pnas.87.7.2521.
Zotero 插件