Prince A M, Brotman B, Inchauspé G, Pascual D, Nasoff M, Hosein B, Wang C Y
Laboratory of Virology and Parasitology, Lindsley F. Kimball Research Institute, New York Blood Center, NY 10021.
J Infect Dis. 1993 Jun;167(6):1296-301. doi: 10.1093/infdis/167.6.1296.
Improved serologic and polymerase chain reaction (PCR)-based tests for hepatitis C virus (HCV) infection provided an opportunity to reexamine a posttransfusion follow-up study done from 1969 to 1972. A total of 213 cardiac surgery patients was prospectively followed after receiving an average of 18 units of blood, 24% of which was from paid donors. Serial sera were tested for antibody to recombinant DNA-derived C100-3 and capsid polypeptides; selected cases were also tested against synthetic peptides derived from different regions of the HCV sequence. PCR and RIBA II immunoblot assays were done on selected sera. Each of 55 probable and 5 of 11 possible hepatitis cases who were seronegative before transfusion seroconverted. Anti-HCV seroconversion also occurred in 6 (4%) of 148 subjects without hepatitis. Among subjects followed > 1 year, PCR positivity persisted in 14 (82%) of 17. If the results of this study can be generalized, all bloodborne non-A, non-B hepatitis may be due to HCV.
针对丙型肝炎病毒（HCV）感染的血清学检测和基于聚合酶链反应（PCR）的检测方法的改进，为重新审视1969年至1972年进行的一项输血后随访研究提供了契机。共有213名心脏手术患者在接受平均18单位血液后接受前瞻性随访，其中24%的血液来自有偿献血者。对系列血清进行了针对重组DNA衍生的C100 - 3和衣壳多肽抗体的检测；部分病例还针对源自HCV序列不同区域的合成肽进行了检测。对选定的血清进行了PCR和RIBA II免疫印迹分析。输血前血清学阴性的55例可能的肝炎病例和11例可能的肝炎病例中的5例发生了血清转化。148名无肝炎的受试者中有6例（4%）也发生了抗HCV血清转化。在随访超过1年的受试者中，17例中有14例（82%）PCR检测持续呈阳性。如果这项研究的结果能够推广，那么所有血源性非甲非乙型肝炎可能都归因于HCV。
