Lipman N S, Corning B F, Saifuddin M
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge 02139.
Lab Anim. 1993 Apr;27(2):134-40. doi: 10.1258/002367793780810360.
Two isolator caging systems were evaluated against challenge with MHV-Y, an enterotropic strain of mouse hepatitis virus. The systems were similar in that they both used an identical shoebox cage equipped with a polycarbonate filter top incorporating a Reemay filter. They differed in that one system supplied HEPA-filtered air through a grommet in the filter lid so that the cage was pressurized slightly. A rack holding 60 cages (30 front and back) was utilized. Thirty cages without filter tops housed one mouse each that had been infected orally with 19,000 ID50 of MHV-Y and an uninfected cagemate. The remaining 30 cages, each housing 2 uninfected mice were divided into 3 groups of 10 cages. Group I cages (controls) had no filter top; Group II cages were equipped with filter tops; and Group III were equipped with filter tops and intracage HEPA-filtered air. The cages housing uninfected mice were interspersed between, above, below and behind cages housing infected mice. The uninfected mice were maintained in contact with the MHV-Y infected mice for 8 weeks. Transmission of MHV-Y was determined serologically by indirect ELISA. All mice housed within the Group I cages (control) seroconverted to MHV, while only 4 mice (2 cages) seroconverted in Group II, and no mice seroconverted in Group III.
对两种隔离笼养系统进行了评估，以抵抗小鼠肝炎病毒的肠道嗜性毒株MHV-Y的攻击。这两种系统的相似之处在于，它们都使用了相同的鞋盒式笼子，配备了带有Reemay过滤器的聚碳酸酯过滤顶盖。它们的不同之处在于，一种系统通过过滤器盖上的索环供应经过高效空气过滤器过滤的空气，从而使笼子略微增压。使用了一个容纳60个笼子（前后各30个）的架子。30个没有过滤顶盖的笼子中，每个笼子饲养一只经口服感染19,000个半数感染剂量MHV-Y的小鼠和一只未感染的同笼小鼠。其余30个笼子，每个笼子饲养2只未感染的小鼠，分为3组，每组10个笼子。第一组笼子（对照组）没有过滤顶盖；第二组笼子配备了过滤顶盖；第三组笼子配备了过滤顶盖和笼内高效空气过滤器过滤的空气。饲养未感染小鼠的笼子穿插在饲养感染小鼠的笼子之间、上方、下方和后面。未感染的小鼠与感染MHV-Y的小鼠接触饲养8周。通过间接酶联免疫吸附测定法血清学检测MHV-Y的传播情况。饲养在第一组笼子（对照组）中的所有小鼠均血清转化为MHV阳性，而第二组中只有4只小鼠（2个笼子）血清转化，第三组中没有小鼠血清转化。
