Benschop R J, Jabaaij L, Oostveen F G, Vingerhoets A J, Kirschbaum C, Duivenvoorden H J, Ballieux R E
University of Utrecht, The Netherlands.
Life Sci. 1993;52(23):1825-34. doi: 10.1016/0024-3205(93)90002-k.
The present report investigated whether percentages of circulating natural killer (NK) cells and NK cell activity (NKCA) are associated with psychological variables. Subjects (n = 95) were selected, based on a combination of low or high scores on questionnaires on daily hassles and self-reported symptoms, to create four extreme groups. NK cell percentages were different between two of the four groups, only when the analysis was not controlled for gender, life style and endocrine parameters. No evidence was found for a relationship between group membership and NKCA. NKCA, however, was found to differ between men and women and to be associated with percentages of NK cells and intracellular levels of cAMP. Furthermore, the hypothesis was tested, that hormone-induced changes in NKCA in vitro are dependent on the individual's current stress profile. To investigate this issue, NKCA was measured after cells had been incubated with hydrocortisone (10(-6) or 10(-7) M) or the beta-adrenergic agonist isoprenaline (10(-5) or 10(-7) M) in vitro. Changes in NKCA were found to be related to plasma adrenaline levels, but no evidence was found for involvement of psychological variables. It is concluded that, in the current setting, there is no association between the combination of scores on the two psychological questionnaires, and NKCA or hormone-induced changes therein.
本报告调查了循环自然杀伤（NK）细胞百分比和NK细胞活性（NKCA）是否与心理变量相关。根据日常烦恼问卷和自我报告症状问卷得分的高低组合，选取了95名受试者，组成四个极端组。只有在未对性别、生活方式和内分泌参数进行分析控制时，四组中的两组之间的NK细胞百分比才存在差异。未发现分组与NKCA之间存在关联。然而，发现NKCA在男性和女性之间存在差异，并且与NK细胞百分比和细胞内cAMP水平相关。此外，还检验了以下假设：体外激素诱导的NKCA变化取决于个体当前的应激状况。为了研究这个问题，在细胞与氢化可的松（10^(-6)或10^(-7) M）或β-肾上腺素能激动剂异丙肾上腺素（10^(-5)或10^(-7) M）体外孵育后，测量了NKCA。发现NKCA的变化与血浆肾上腺素水平有关，但未发现心理变量参与其中的证据。得出的结论是，在当前情况下，两份心理问卷得分的组合与NKCA或其中激素诱导的变化之间没有关联。
