Nieman D C, Ahle J C, Henson D A, Warren B J, Suttles J, Davis J M, Buckley K S, Simandle S, Butterworth D E, Fagoaga O R
Department of Health, Appalachian State University, Boone, North Carolina 28608, USA.
J Appl Physiol (1985). 1995 Sep;79(3):748-55. doi: 10.1152/jappl.1995.79.3.748.
The effect of 2.5 h of treadmill running at 75.6 +/- 0.9% maximal O2 uptake (VO2max) on natural killer (NK) cell cytotoxic activity (NKCA) was investigated in 22 experienced marathon runners (VO2max 57.9 +/- 1.1 ml.kg-1.min-1, age 38.7 +/- 1.5 yr). Blood samples were taken before (0715) and immediately after exercise (1000), with three more samples taken during 6 h of recovery (1130, 1300, and 1600). Ten sedentary controls (VO2max 34.7 +/- 1.0 ml.kg-1.min-1, age 45.3 +/- 2.3 yr) sat in the laboratory during testing and had their blood sampled at the same time points. The pattern of change in NKCA over time was significantly different between groups [F(4,27) = 6.53; P = 0.001], with the runner's NKCA dropping 51-61% below preexercise levels throughout 6 h of recovery. Preincubation of blood mononuclear cells in vitro with indomethacin had no effect on the difference in pattern of change in NKCA between groups [F(4,17) = 8.59; P = 0.001] and did not attenuate the postexercise reduction in the runners. When NKCA was adjusted on a per-NK cell basis, group differences and the postexercise decline in NKCA were eliminated [F(4,80) = 0.65; P = 0.63]. Serum cortisol and plasma epinephrine in the runners were elevated relative to control subjects during recovery from exercise, but no significant correlation with changes in NK cells or NKCA was found. These data indicate that NKCA is decreased significantly during recovery from 2.5 h of running due to a numerical redistribution of NK cells.
在22名经验丰富的马拉松运动员（最大摄氧量[VO₂max]为57.9±1.1 ml·kg⁻¹·min⁻¹，年龄38.7±1.5岁）中，研究了以最大摄氧量的75.6±0.9%进行2.5小时跑步机跑步对自然杀伤（NK）细胞细胞毒性活性（NKCA）的影响。在运动前（0715）和运动后立即（1000）采集血样，在恢复的6小时内（1130、1300和1600）再采集三个血样。10名久坐不动的对照组人员（VO₂max为34.7±1.0 ml·kg⁻¹·min⁻¹，年龄45.3±2.3岁）在测试期间坐在实验室，并在相同时间点采集血样。两组之间NKCA随时间变化的模式有显著差异[F(4,27)=6.53；P = 0.001]，在整个6小时的恢复过程中，跑步者的NKCA比运动前水平下降了51%-61%。体外将血液单核细胞与吲哚美辛预孵育对两组之间NKCA变化模式的差异没有影响[F(4,17)=8.59；P = 0.001]，也没有减弱跑步者运动后的NKCA降低。当以每个NK细胞为基础调整NKCA时，组间差异和运动后NKCA的下降被消除[F(4,80)=0.65；P = 0.63]。在运动恢复期间，跑步者的血清皮质醇和血浆肾上腺素相对于对照组有所升高，但未发现与NK细胞或NKCA的变化有显著相关性。这些数据表明，由于NK细胞的数量重新分布，在2.5小时跑步后的恢复过程中NKCA显著降低。
