Hu L F, Chen F, Zheng X, Ernberg I, Cao S L, Christensson B, Klein G, Winberg G
Department of Tumor Biology, Karolinska Institute, Sweden.
Oncogene. 1993 Jun;8(6):1575-83.
Two isolates of the EBV-LMP1 gene were compared for their ability to induce phenotypic changes in a non-tumorigenic human keratinocyte line, Rhek-1, immortalized with an adenovirus 12-SV40 hybrid virus. One isolate, designated B-LMP1, was derived from B95-8, a B-cell line of marmoset origin, that carries a viral strain from a mononucleosis patient. The other, designated C-LMP1, originated from a nude mouse passaged Chinese NPC tumor, CAO. Both types of transfectants were less serum dependent than the non-transfected and the vector-transfected controls. The ability to grow on low serum increased with increasing LMP1 expression. All transfectants were more highly clonable than the non-transfected or vector-transfected controls. Clonability in soft agarose increased with increasing LMP1 expression. Nine of 24 C-LMP1 transfectants produced tumors in SCID mice. Seven of them grew invasively into the surrounding tissue. Only one of 12 B-LMP1 transfected Rhek-1 clones was tumorigenic. It did not grow invasively. All tumorigenic transfectants expressed LMP1 at high or moderate levels. All tumors were found to express LMP1. Transfectants with low LMP1 expression did not produce tumors. The untransfected Rhek-1 cells and six vector control clones failed to produce tumors.
比较了EBV-LMP1基因的两个分离株在由腺病毒12-SV40杂交病毒永生化的非致瘤性人角质形成细胞系Rhek-1中诱导表型变化的能力。一个分离株命名为B-LMP1，来源于B95-8，一种源自狨猴的B细胞系，它携带来自一名单核细胞增多症患者的病毒株。另一个命名为C-LMP1，源自经裸鼠传代的中国鼻咽癌肿瘤CAO。两种转染子都比未转染和载体转染的对照对血清的依赖性更低。在低血清中生长的能力随着LMP1表达的增加而增强。所有转染子都比未转染或载体转染的对照更易于克隆。在软琼脂糖中的克隆能力随着LMP1表达的增加而增强。24个C-LMP1转染子中有9个在SCID小鼠中产生了肿瘤。其中7个侵袭性生长到周围组织中。12个B-LMP1转染的Rhek-1克隆中只有1个具有致瘤性。它没有侵袭性生长。所有致瘤性转染子都高或中度表达LMP1。所有肿瘤都被发现表达LMP1。LMP1表达低的转染子没有产生肿瘤。未转染的Rhek-1细胞和6个载体对照克隆未能产生肿瘤。
