Pacchioni D, Papotti M, Andorno E, Bonino F, Mondardini A, Oliveri F, Brunetto M, Bussolati G, Negro F
Department of Biomedical Sciences and Human Oncology, University of Turin School of Medicine, Italy.
J Surg Oncol Suppl. 1993;3:14-7. doi: 10.1002/jso.2930530505.
Estrogen receptor mRNA was detected by a non-radioactive in situ hybridization assay in tumor and non-neoplastic liver tissues. A synthetic oligonucleotide complementary to the human estrogen receptor mRNA was 3'-labeled with digoxigenin-deoxyuridine triphosphate (dUTP). Hybrids were revealed by an alkaline phosphatase-conjugated anti-digoxigenin antibody. Fourteen primary hepatocellular carcinoma tissues (and one metastatic) were obtained at surgery from 15 patients. The corresponding non-neoplastic liver tissues were available in 13 cases. The estrogen receptor mRNA was detected in 11 tumorous and 7 non-tumorous liver specimens. The staining was cytoplasmic and involved the majority of transformed hepatocytes, whereas a less widespread and weaker signal was found in normal hepatocytes. Within non-neoplastic tissue, bile duct epithelial cells could also be occasionally stained, whereas other cell types, such as vasal endothelial cells, were negative. Appropriate controls established the specificity of the reaction. Detection of the estrogen receptor protein by immunohistochemistry in these same specimens was invariably negative. This in situ hybridization assay can therefore be used as a complementary tool to evaluate the estrogen receptor expression within liver cancer.
采用非放射性原位杂交分析法在肿瘤及非肿瘤性肝组织中检测雌激素受体mRNA。与人类雌激素受体mRNA互补的合成寡核苷酸用洋地黄毒苷-脱氧尿苷三磷酸（dUTP）进行3'标记。通过碱性磷酸酶偶联的抗洋地黄毒苷抗体显示杂交体。15例患者手术获取了14例原发性肝细胞癌组织（1例为转移癌）。13例患者有相应的非肿瘤性肝组织。在11例肿瘤性肝标本和7例非肿瘤性肝标本中检测到雌激素受体mRNA。染色位于细胞质，累及大多数转化的肝细胞，而在正常肝细胞中信号分布较窄且较弱。在非肿瘤组织中，胆管上皮细胞偶尔也可被染色，而其他细胞类型，如血管内皮细胞呈阴性。适当的对照确定了反应的特异性。在这些相同标本中通过免疫组织化学检测雌激素受体蛋白始终为阴性。因此，这种原位杂交分析法可作为评估肝癌内雌激素受体表达的一种补充工具。
