Williams J W, Carlson D L, Gadson R G, Rollins-Smith L, Williams C S, McKinnell R G
Department of Biology, Tuskegee University, AL.
Cytogenet Cell Genet. 1993;64(1):18-22. doi: 10.1159/000133551.
To provide a cytogenetic marker for nuclear transplantation experiments, triploid Rana pipiens embryos were produced. These embryos were injected with Lucké tumor herpesvirus. The chromosome profile of a renal carcinoma that developed in one of these triploid embryos was compared to the chromosomal profiles of a naturally occurring diploid renal carcinoma and a diploid renal tumor maintained as serial anterior eye chamber allografts for over 7 yr. Examination of Ag-NOR-stained chromosome spreads from the putative triploid and naturally occurring putative diploid tumor revealed the expected results. The vast majority of the chromosome spreads, 54/57 (95%) and 6/7 (86%), respectively, displayed euploid chromosome and Ag-NOR profiles: 3N = 39 with three Ag-NORs at the secondary constrictions in the long arm of chromosome 10 (10q) and 2N = 26 with two Ag-NORs in 10q. Chromosome profiles from the long-term allografted tumor were highly aneuploid (82%) and, based on their Ag-NOR content, displayed variations in their 2N, 3N, and 4N numbers. These data indicate that the majority of recently transformed triploid Lucké tumor cells can provide donor nuclei suitable for the characterization of developmental potential.
为了给核移植实验提供一个细胞遗传学标记，制备了三倍体牛蛙胚胎。向这些胚胎注射了勒克氏肿瘤疱疹病毒。将其中一个三倍体胚胎中发生的肾癌的染色体图谱与自然发生的二倍体肾癌以及作为连续前房同种异体移植维持了7年以上的二倍体肾肿瘤的染色体图谱进行了比较。对来自假定三倍体和自然发生的假定二倍体肿瘤的银染核仁组织区（Ag-NOR）染色体铺展进行检查，得到了预期结果。绝大多数染色体铺展，分别为54/57（95%）和6/7（86%），显示出整倍体染色体和Ag-NOR图谱：3N = 39，在10号染色体长臂（10q）的次缢痕处有三个Ag-NOR；2N = 26，在10q有两个Ag-NOR。长期同种异体移植肿瘤的染色体图谱高度非整倍体（82%），并且根据其Ag-NOR含量，显示出其二倍体、三倍体和四倍体数量的变化。这些数据表明，大多数最近转化的三倍体勒克氏肿瘤细胞可以提供适合于发育潜能表征的供体细胞核。
