Beaudet A, Descarries L
Brain Res. 1979 Jan 12;160(2):231-43. doi: 10.1016/0006-8993(79)90421-9.
Intensely labeled nerve cell bodies were identified by radioautography within the pars ventralis of nucleus dorsomedialis hypothalami (hdv), following intraventricular perfusion with 10(-5) or 10(-4) M tritiated serotonin [3H]5-HT in adult rats pretreated with a monoamine oxidase inhibitor. This selective reaction, which involved approximately 1000 neurons on each side of the third ventricle, was unaltered by concomitant administration of 10(-3) M non-radioactive norepinephrine, and was absent after intraventricular injection of 10(-5) or 10(-4) M tritiated norepinephrine. The 3H-labeled 5-HT nerve cell bodies were loosely grouped within the inner and caudal half of the hdv, and appeared morphologically similar to the unreactive neurons among which they were interspersed. Within the same region, numerous labeled axonal varicosities were also detected, which were never found in synaptic contact with the reactive cells. If the 3H-labeled 5-HT neurons contain endogenous 5-HT, they might constitute an intrinsic source of 5-HT innervation in the adult rat hypothalamus.
在成年大鼠中，先用单胺氧化酶抑制剂预处理，然后经脑室灌注10(-5)或10(-4)M的氚标记血清素[3H]5-HT，通过放射自显影术在丘脑背内侧核腹侧部（hdv）内鉴定出标记强烈的神经细胞体。这种选择性反应涉及第三脑室每侧约1000个神经元，同时给予10(-3)M非放射性去甲肾上腺素时该反应未改变，而在脑室注射10(-5)或10(-4)M氚标记去甲肾上腺素后则不存在这种反应。3H标记的5-HT神经细胞体松散地聚集在hdv的内侧和尾侧半部内，形态上与它们散布于其中的无反应神经元相似。在同一区域内，还检测到许多标记的轴突膨体，从未发现它们与反应性细胞形成突触接触。如果3H标记的5-HT神经元含有内源性5-HT，它们可能构成成年大鼠下丘脑5-HT神经支配的一个内在来源。
