Lelias J M, Kaghad M, Rodriguez M, Chalon P, Bonnin J, Dupre I, Delpech B, Bensaid M, LeFur G, Ferrara P
Laboratoire de Biologie Moleculaire, Sanofi Elf Biorecherches, Labege, France.
FEBS Lett. 1993 Jun 14;324(2):127-30. doi: 10.1016/0014-5793(93)81377-c.
We report the molecular cloning of a beta 3-adrenergic receptor [beta 3-AR] cDNA from human brown adipose tissue. The cDNA-encoded protein is identical to the previously cloned beta 3-AR but with 6 additional amino acids at the C-terminus. The C-terminus is shared by the beta 3 receptors expressed in human neuroblastoma cells [SK-N-MC] [Mol. Pharmacol. 42 (1992) 964-970]. Furthermore, using a polymerase chain reaction strategy we have cloned and sequenced the beta 3-AR introns. Sequence analysis demonstrates that the human beta 3-AR gene comprises at least 3 exons and 2 introns and that the most abundant beta 3-AR transcripts encode a protein with an exon 3-derived C-terminus. Interestingly, although a similar organization has been found in rodent genes, the rat beta 3-AR transcripts encode a receptor with an exon 2-derived C-terminus.
我们报道了从人棕色脂肪组织中克隆β3-肾上腺素能受体[β3-AR] cDNA的过程。该cDNA编码的蛋白与先前克隆的β3-AR相同，但在C末端有6个额外的氨基酸。人神经母细胞瘤细胞[SK-N-MC]中表达的β3受体也具有相同的C末端[《分子药理学》42(1992)964 - 970]。此外，我们采用聚合酶链反应策略克隆并测序了β3-AR内含子。序列分析表明，人β3-AR基因至少包含3个外显子和2个内含子，且最丰富的β3-AR转录本编码一种具有源自外显子3的C末端的蛋白。有趣的是，尽管在啮齿动物基因中发现了类似的结构，但大鼠β3-AR转录本编码的受体具有源自外显子2的C末端。
