Eiden J J, Hirshon C
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Virology. 1993 Jan;192(1):154-60. doi: 10.1006/viro.1993.1017.
The complete nucleic acid sequence was determined for the largest genomic segment of the IDIR strain of group B rotavirus and compared with RNA polymerase genes of rotavirus groups A and C as well as other RNA viruses. IDIR gene 1 contained 3509 bases with a single, long open reading frame which encoded a deduced polypeptide of 1159 amino acids (MW = 131.6 kDa; pl 8.851). The deduced amino acid sequence of IDIR gene 1 shared 50% similar sequences and 27.6% identical sequences with VP1 of the RF strain of group A rotavirus. IDIR gene 1 also contained 45.4% similar and 26.5% identical amino acid sequences in comparison with gene 1 of the Cowden strain of group C rotavirus. Amino acids 643-689 of IDIR gene 1 corresponded to the conserved viral RNA polymerase domains, "SG . . . T . . . NS . . N" and "GDD." Within these domains, group A, B, and C rotaviruses displayed substantial homologies that were not shared with other RNA viruses. These sequences indicated the presence of highly conserved structural or functional components among groups of rotaviruses which were otherwise quite heterogeneous. The identification of rotavirus-specific residues within RNA polymerase sequence may prove valuable in devising strategies aimed at the control of rotavirus replication and infection.
测定了B组轮状病毒IDIR株最大基因组片段的完整核酸序列，并将其与A组和C组轮状病毒的RNA聚合酶基因以及其他RNA病毒进行了比较。IDIR基因1包含3509个碱基，有一个单一的长开放阅读框，编码一个由1159个氨基酸组成的推导多肽（分子量 = 131.6 kDa；等电点8.851）。IDIR基因1的推导氨基酸序列与A组轮状病毒RF株的VP1有50%的相似序列和27.6%的相同序列。与C组轮状病毒考登株的基因1相比，IDIR基因1也含有45.4%的相似氨基酸序列和26.5%的相同氨基酸序列。IDIR基因1的643 - 689位氨基酸对应于保守的病毒RNA聚合酶结构域“SG...T...NS...N”和“GDD”。在这些结构域内，A组、B组和C组轮状病毒显示出与其他RNA病毒不共有的大量同源性。这些序列表明，轮状病毒组之间存在高度保守的结构或功能成分，而这些轮状病毒组在其他方面是相当异质的。在RNA聚合酶序列中鉴定轮状病毒特异性残基可能在设计控制轮状病毒复制和感染的策略方面具有重要价值。
