斑蝥素诱导的大鼠炎症模型局部抗炎活性评估

Assessment of topical anti-inflammatory activity in rats with cantharidin-induced inflammation.

作者信息

Boris A, Hurley J F

出版信息

J Invest Dermatol. 1977 Mar;68(3):161-4. doi: 10.1111/1523-1747.ep12492482.

Abstract

Topical application of 400 mug of cantharidin to the rat's ear caused an approximate doubling in the mean weight of uniform ear punch samples when compared to vehicle-treated controls at 72 hr, and produced a maximal response at 7 days. Dexamethasone reduced the increase in weight when applied topically, but was ineffective when given subcutaneously or orally at the same doses. Hydrocortisone, prednisolone, triamcinolone, betamethasone, flurometholone, paramethasone acetate, fluocinolone acetonide, fluocinonide, and flurandrenolide showed significant suppression of cantharidin-induced inflammation. Cholesterol, diphenhydramine, tripelennamine, chlorpheniramine, promethazine, cyproheptadine, epinephrine, phenylephrine, alpha-tocopherol, indomethacin, and bufexamac were inactive. It is suggested that the procedure employed may be useful in the screening and evaluation of topical anti-inflammatory agents.

摘要

与赋形剂处理的对照组相比，在72小时时，将400微克斑蝥素局部应用于大鼠耳部，使均匀耳打孔样本的平均重量增加了约一倍，并在7天时产生最大反应。地塞米松局部应用时可减轻重量增加，但以相同剂量皮下或口服给药时无效。氢化可的松、泼尼松龙、曲安奈德、倍他米松、氟米龙、醋酸帕拉米松、醋酸氟轻松、氟轻松和氟氢缩松均显示出对斑蝥素诱导的炎症有显著抑制作用。胆固醇、苯海拉明、曲吡那敏、氯苯那敏、异丙嗪、赛庚啶、肾上腺素、去氧肾上腺素、α-生育酚、吲哚美辛和丁苯羟酸无活性。提示所采用的方法可能有助于局部抗炎药的筛选和评价。