Takada H, Kishimoto C, Kuroki Y, Matsushita I, Hiraoka Y, Kurokawa M, Ochiai H, Sasayama S
Second Department of Internal Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Heart Vessels. 1993;8(2):59-66. doi: 10.1007/BF01744384.
The aim of this study is to test the therapeutic efficacy of immunomodulation with lobenzarit disodium (CCA) upon coxsackievirus B3 (CB3) myocarditis. Two-week-old C3H/He mice were inoculated with 10(3) plaque-forming units of CB3. CCA, 2.5 mg/kg per day, was administered subcutaneously daily on days 0-14 (Experiment I; group 2) and days 14-28 (Experiment II; group 4). Both treated groups were compared to infected controls (groups 1 and 3). For the analysis of splenic lymphocyte subsets, additional mice in untreated and treated groups were killed on day 7, and the percentages of Thy 1.2 (CD3), L3T4 (CD4) and, Ly 2 (CD8) subsets were analyzed by laser flow cytometry (Experiment III). In Experiment I, the survival rate did not differ significantly between groups 1 and 2. Cellular infiltration in the CCA group was less severe. Myocardial virus titers and serum neutralizing antibody titers did not differ significantly between the two groups. In Experiment II, the survival rates between the two groups did not differ significantly. Myocardial necrosis in the CCA group was less severe compared to the control. In Experiment III, the percentages of Thy 1.2 (CD3) and L3T4 subsets (CD4) of the treated group were significantly higher than those of the control group. Thus, CCA increased splenic T cells and improved cardiac pathology in acute murine CB3 myocarditis.
本研究的目的是测试氯苯扎利二钠（CCA）免疫调节对柯萨奇病毒B3（CB3）心肌炎的治疗效果。给两周大的C3H/He小鼠接种10³个噬斑形成单位的CB3。在第0 - 14天（实验I；第2组）和第14 - 28天（实验II；第4组）每天皮下注射2.5mg/kg的CCA。将两个治疗组与感染对照组（第1组和第3组）进行比较。为了分析脾脏淋巴细胞亚群，在第7天处死未治疗组和治疗组中的额外小鼠，并通过激光流式细胞术分析Thy 1.2（CD3）、L3T4（CD4）和Ly 2（CD8）亚群的百分比（实验III）。在实验I中，第1组和第2组的存活率没有显著差异。CCA组的细胞浸润较轻。两组之间的心肌病毒滴度和血清中和抗体滴度没有显著差异。在实验II中，两组之间的存活率没有显著差异。与对照组相比，CCA组的心肌坏死较轻。在实验III中，治疗组的Thy 1.2（CD3）和L3T4亚群（CD4）的百分比显著高于对照组。因此，CCA增加了脾脏T细胞，并改善了急性小鼠CB3心肌炎的心脏病理状况。
