Kurihara T, Yokoe T, Iino Y, Ishida T, Morishita Y
Second Department of Surgery, Gunma University School of Medicine, Maebashi, Japan.
Nihon Geka Gakkai Zasshi. 1993 Mar;94(3):277-84.
The usefulness of the measurement of nuclear DNA content by fine needle aspiration biopsy was evaluated through a comparative study of fine needle aspirated and tissue samples. Nuclear DNA content was measured in patients with 28 primary and 2 metastatic lesions of breast carcinoma using fluorescent cytophotometry with Feulgen stain. The rate of correspondence on DNA ploidy pattern of aspirates and tissue samples was 86.7%. There was a significant correlation with DNA index (DI) (R = 0.85, p < 0.001) and S-phase fraction (%S) (R = 0.79, p < 0.001). The rate of correspondence of DI subgroups (3 aneuploid subgroups and diploid) was 80.8%. No tumor size affected values of DI and %S of aspirates. However, %S of papillotubular carcinoma showed small difference compared with values of tissue samples. Nuclear DNA content of fine needle aspirated samples had a significant correlation with that of tissue samples, providing the estimation of the original ploidy pattern and DI. Fine needle aspiration biopsy is an easy technique and able to be carried out repeatedly. Thus, the measurement of nuclear DNA content using aspirates is useful for broad practical purposes.
通过对细针穿刺抽吸样本和组织样本的比较研究，评估了细针穿刺活检测量核DNA含量的实用性。使用福尔根染色荧光细胞光度法，对28例原发性乳腺癌和2例转移性乳腺癌患者的细针穿刺抽吸样本和组织样本进行核DNA含量测量。抽吸样本与组织样本的DNA倍体模式对应率为86.7%。与DNA指数（DI）（R = 0.85，p < 0.001）和S期分数（%S）（R = 0.79，p < 0.001）存在显著相关性。DI亚组（3个非整倍体亚组和二倍体）的对应率为80.8%。肿瘤大小对抽吸样本的DI和%S值无影响。然而，乳头管状癌的%S与组织样本的值相比差异较小。细针穿刺抽吸样本的核DNA含量与组织样本的核DNA含量存在显著相关性，可用于估计原始倍体模式和DI。细针穿刺活检是一种简单的技术，能够重复进行。因此，使用抽吸样本测量核DNA含量对于广泛的实际应用是有用的。
