Niezabitowski A, Lackowska B, Gruchala A, Ryś J, Jaszcz A, Szklarski W, Wasilewska A, Herman K
Department of Pathology, Oncology Center, Cracow, Poland.
Gen Diagn Pathol. 1996 Jun;142(1):33-9.
In 221 consecutive, unselected palpable breast lesions, evaluated by aspiration biopsy, flow cytometric DNA analysis was performed on cell material remaining in needle and syringe after smears had been prepared. DNA histograms were obtained in 186 cases, whereas in 44 samples the cell number was too low for detailed estimation of cell kinetics. Aneuploidy was observed in 71 of 90 samples where cancer cells were detected by cytology, in four of 20 samples where breast cancer could only be suspected and in 12 of those 76 where no cancer cells occurred in cytologic specimens. The average S-phase and S+G2M values were higher in aneuploid than in diploid cell samples. In the group of 40 cases (34 breast cancers, 6 benign lesions) comparative analysis of DNA histograms obtained on aspirates and tissue fragments revealed a concordance in 88.9%. Modified radical mastectomy was performed in 41 breast cancers and in 33 cases ductal infiltrating carcinomas were recognized by histology and scored according to Bloom-Richardson score. Cell features assessed by cytology in these cases did not correlate with histology. In most cases, however, nuclear polymorphism and numerous naked nuclei were present in cytologic specimens. These alterations, characterized as score 3, reflect values of DNA-index in compartment of 1.4-1.8.
对221例连续的、未经选择的可触及乳腺病变进行针吸活检评估，在制备涂片后，对针管和注射器中剩余的细胞材料进行流式细胞术DNA分析。186例获得了DNA直方图，而44个样本的细胞数量过少，无法详细评估细胞动力学。在细胞学检测到癌细胞的90个样本中，71个观察到非整倍体；在仅怀疑为乳腺癌的20个样本中，4个观察到非整倍体；在细胞学标本中未发现癌细胞的76个样本中，12个观察到非整倍体。非整倍体细胞样本的平均S期和S+G2M值高于二倍体细胞样本。在40例（34例乳腺癌，6例良性病变）中，对针吸物和组织碎片获得的DNA直方图进行比较分析，一致性为88.9%。41例乳腺癌患者接受了改良根治性乳房切除术，33例经组织学确诊为导管浸润癌，并根据Bloom-Richardson评分进行评分。这些病例中通过细胞学评估的细胞特征与组织学不相关。然而，在大多数病例中，细胞学标本中存在核多形性和大量裸核。这些改变被归类为3分，反映了DNA指数在1.4-1.8区间的值。
