Gold L W, Massry S G, Friedler R M
J Lab Clin Med. 1977 Mar;89(3):554-9.
The renal handling of glucose was evaluated in six dogs before and after chronic phosphate depletion of 51 to 112 days' duration. Phosphate depletion was produced by feeding the dogs with phosphate-restricted diet and administration of Al(OH)3. With phosphate depletion, serum phosphate fell from 3.5 +/- 0.36 (S.E.) to 0.5 +/- 0.19 mg./dl. Both TmG and TmG/GFR fell significantly during phosphate depletion. TmG/GFR was 4.0 +/- 0.41 mg./ml. before phosphate depletion and 2.9 +/- 0.19 mg./ml. after phosphate depletion. In four phosphate-depleted dogs TmG/GFR fell precipitously after glucose loading for more than 200 minutes, and practically all of the filtered glucose was excreted in the urine. It is possible that the levels of cytosolic adenine nucleotides which are required for energy of the renal cell may be reduced during phosphate depletion. This may underlie the fall in TmG and may explain the precipitous fall in TmG when the transport mechanism for glucose was operating at maximum capacity for a prolonged period of time.
在6只犬身上评估了为期51至112天的慢性磷酸盐耗竭前后的肾脏对葡萄糖的处理情况。通过给犬喂食低磷饮食并给予氢氧化铝来造成磷酸盐耗竭。随着磷酸盐耗竭，血清磷酸盐从3.5±0.36（标准误）降至0.5±0.19毫克/分升。在磷酸盐耗竭期间，肾小管葡萄糖最大重吸收量（TmG）和TmG/肾小球滤过率（GFR）均显著下降。磷酸盐耗竭前TmG/GFR为4.0±0.41毫克/毫升，磷酸盐耗竭后为2.9±0.19毫克/毫升。在4只磷酸盐耗竭的犬中，葡萄糖负荷超过200分钟后TmG/GFR急剧下降，几乎所有滤过的葡萄糖都随尿排出。在磷酸盐耗竭期间，肾细胞能量所需的胞质腺嘌呤核苷酸水平可能降低。这可能是TmG下降的基础，并且可以解释当葡萄糖转运机制长时间以最大容量运行时TmG的急剧下降。
