Morace G, Pisani G, Beneduce F, Divizia M, Panà A
Laboratory of Virology, Istituto Superiore di Sanità, Rome, Italy.
Virus Res. 1993 May;28(2):187-94. doi: 10.1016/0168-1702(93)90135-a.
Two strains of hepatitis A virus (HAV) were isolated in cell culture and found to induce a cytopathic effect at early passages. The nucleotide sequences of the 5' non-translated region (5'NTR) and of genes 2B, 2C, 3A and 3B were determined for these strains and found to contain mutations similar to those detected in cell-culture adapted variants of HAV strain HM175. In addition, gene 3A shows a deletion of three aspartic acid residues near the N-terminus of the polypeptide. In combination with variations in the 5'NTR and in genes 2B and 2C, the absence of an aspartic acid residue in position 4 of gene 3A of three cytopathic clones of HM175 suggests a possible role of the 3A protein in determining the cytopathic phenotype.
在细胞培养中分离出两株甲型肝炎病毒（HAV），发现在传代早期可诱导细胞病变效应。测定了这些毒株5'非翻译区（5'NTR）以及基因2B、2C、3A和3B的核苷酸序列，发现它们含有与在HAV毒株HM175的细胞培养适应变体中检测到的类似突变。此外，基因3A在多肽N端附近缺失了三个天冬氨酸残基。结合5'NTR以及基因2B和2C的变异，HM175的三个细胞病变克隆的基因3A第4位天冬氨酸残基缺失表明3A蛋白在决定细胞病变表型方面可能发挥作用。
