Brack K, Frings W, Dotzauer A, Vallbracht A
Department of Virology, University of Bremen, Germany.
J Virol. 1998 Apr;72(4):3370-6. doi: 10.1128/JVI.72.4.3370-3376.1998.
A cytopathogenic variant of hepatitis A virus (HAV(cyt/HB1.1)) was isolated from persistently infected BS-C-1 cells by serial passages in FRhK-4 cells. This virus shows a rapid replication pattern and high final titers are obtained, which are main characteristics of cytopathogenic HAVs. Sequencing of the nontranslated regions and the coding regions for 2ABC and 3AB revealed that mutations are distributed all over these regions and that certain mutated sites correspond to those in other cytopathogenic HAV variants. Investigating the mechanisms causing the cytopathic effect in FRhK-4 cells infected with this variant, we found that an apoptotic reaction takes place.
通过在FRhK-4细胞中连续传代，从持续感染的BS-C-1细胞中分离出甲型肝炎病毒的细胞病变变体（HAV(cyt/HB1.1)）。该病毒呈现快速复制模式，并能获得高终末滴度，这是细胞病变型甲型肝炎病毒的主要特征。对非翻译区以及2ABC和3AB编码区的测序表明，突变分布在这些区域的各处，且某些突变位点与其他细胞病变型甲型肝炎病毒变体中的位点相对应。在研究感染该变体的FRhK-4细胞中导致细胞病变效应的机制时，我们发现发生了凋亡反应。
