Orfanos A P, Murphey W H, Guthrie R
J Lab Clin Med. 1977 Mar;89(3):659-65.
A simple microfluorometric procedure for the measurement of protoporphyrin in erythrocytes (EPP) is described. The technique, which can be performed in less than 4 minutes with portable equipment, utilizes 40 mul of capillary blood and only one reagent (94 percent acidified ethanol) for the extraction. The results obtained by this micromethod are in close agreement with those of the ethyl acetate/hydrochloric acid double-extraction methods. The EPP concentration increases markedly when the blood lead level is increased, and the test can be used to screen children for lead poisoning. This new micromethod also gives reproducible results when dried blood spots on filter paper are used instead of liquid blood.
本文描述了一种用于测量红细胞中原卟啉（EPP）的简单微量荧光测定方法。该技术使用便携式设备，操作时间不到4分钟，只需40微升毛细血管血和一种试剂（94%酸化乙醇）进行提取。这种微量方法获得的结果与乙酸乙酯/盐酸双提取法的结果非常吻合。当血铅水平升高时，EPP浓度会显著增加，该测试可用于筛查儿童铅中毒。当使用滤纸上的干血斑代替液体血液时，这种新的微量方法也能得到可重复的结果。
