Lamola A A, Eisinger J, Blumberg W E, Kometani T, Burnham B F
J Lab Clin Med. 1977 Apr;89(4):881-90.
An elevated zinc protoporphyrin (ZPP) level in blood is indicative of lead poisoning (as well as iron-deficiency anemia) and is the basis for certain fluorometric screening tests. The calibration of such test protocols and instruments requires ways to reliably determine absolute ZPP concentrations in blood. Two such methods are described: one is an ethanol extraction method and the other a fluorometric addition method using detergent hemolyzed blood. A careful determination of the molar extinction coefficient of ZPP gives EmM=161 in pyridine. Corrections are offered for a previously published protocol for determining the ZPP concentration from the fluorescence intensity of diluted blood. The choice of units for expressing the ZPP concentration in blood is discussed, as is the absorbance correction in quantitative fluorometry of samples which are not optically thin at the excitation wavelength.
血液中锌原卟啉（ZPP）水平升高表明铅中毒（以及缺铁性贫血），是某些荧光筛查测试的基础。此类测试方案和仪器的校准需要可靠测定血液中绝对ZPP浓度的方法。本文描述了两种这样的方法：一种是乙醇萃取法，另一种是使用去污剂溶血血液的荧光添加法。仔细测定ZPP的摩尔消光系数得出在吡啶中的EmM = 161。针对先前发表的从稀释血液的荧光强度测定ZPP浓度的方案提供了校正。讨论了表示血液中ZPP浓度的单位选择，以及在激发波长下光学厚度不足的样品定量荧光测定中的吸光度校正。
