D'Alessandro J S, Yetz-Aldape J, Wang E A
Genetics Institute Inc., Cambridge, MA 02140.
Growth Factors. 1994;11(1):53-69. doi: 10.3109/08977199409015051.
Serum-free mouse embryo (SFME) cells express Glial Fibrillary Acidic Protein (GFAP), a specific marker of the astrocyte lineage, when treated with either Transforming Growth Factor Beta (TGF-beta) or calf serum. We examined the effects of the related Bone Morphogenetic Proteins (BMPs) which are expressed in the developing murine nervous system. Treatment with the heterodimers BMP-2/6 and 2/7 followed by the homodimers BMP-2, 4, 5, 6, and 7 induced higher levels of GFAP in these cells than either TGF-beta 1 or activin when tested at the same concentration. The BMP-induced cells resembled classically described astrocytes and were characterized by antibody markers as type 1 and type 2. In addition, these astrocytes also showed increased levels of the cell adhesion molecules CD44 and neural cell adhesion molecule (N-CAM), both known to be expressed by this cell type. These data clearly demonstrate that the BMPs function as differentiation factors as well as regulators of adhesion molecule expression for cells of the astrocyte lineage and suggest a key role in glial development in the nervous system.
无血清小鼠胚胎（SFME）细胞在使用转化生长因子β（TGF-β）或小牛血清处理时，会表达胶质纤维酸性蛋白（GFAP），这是星形胶质细胞谱系的一种特异性标志物。我们研究了在发育中的小鼠神经系统中表达的相关骨形态发生蛋白（BMP）的作用。当以相同浓度进行测试时，用异二聚体BMP-2/6和2/7以及随后的同二聚体BMP-2、4、5、6和7处理这些细胞，会诱导出比TGF-β1或激活素更高水平的GFAP。BMP诱导的细胞类似于经典描述的星形胶质细胞，并通过抗体标志物鉴定为1型和2型。此外，这些星形胶质细胞还显示出细胞粘附分子CD44和神经细胞粘附分子（N-CAM）水平的增加，已知这两种分子均由这种细胞类型表达。这些数据清楚地表明，BMP作为分化因子以及星形胶质细胞谱系细胞粘附分子表达的调节剂发挥作用，并提示其在神经系统胶质发育中起关键作用。
