Bazzano G, Terezakis N, Attia H, Bazzano A, Dover R, Fenton D, Mandir N, Celleno L, Tamburro M, Jaconi S
Department of Dermatology, Tulane University Medical School, New Orleans, Louisiana.
J Invest Dermatol. 1993 Jul;101(1 Suppl):138S-142S. doi: 10.1111/1523-1747.ep12363264.
It has been demonstrated that topical application of all-trans retinoic acid and other retinoids can alter the hair-growth cycle in the C3H mouse model. The anagen phase is prolonged and the telogen phase is shortened. This effect is similar to the effect of minoxidil on the hair-cycle dynamics in this animal model. The levels of cellular retinoic acid binding protein measured by radioreceptor assay in whole skin of C3H mice were higher during anagen and lower during telogen. Topical application of certain retinoids caused elevated levels of cellular retinoic acid-binding protein (cRABP) in the whole skin homogenates during both phases of the cycle. Of the retinoids tested, those most effective in altering the levels of cRABP in the skin of the mice were also capable of significantly altering the hair-cycle dynamics. There appeared to be a relationship between the ability of retinoid to increase cRABP, increase 3H-thymidine incorporation, and alter the dynamics of the hair cycle. Only cRABP-II is detectable in human cultured dermal fibroblasts and dermal papilla cells. Dermal fibroblasts showed higher amounts of cRABP-II as compared to dermal papilla cells. The difference in cRABP-II expression might explain a distinct response to RA by these two cell populations. Whether the difference in expression of cRABP-II might be of physiologic importance remains to be determined. Treatment of human dermal papilla cells in culture with retinoic acid does not appear to affect proliferation, at least at the doses tested.
已经证明，在C3H小鼠模型中局部应用全反式维甲酸和其他类视黄醇可以改变毛发生长周期。生长期延长，休止期缩短。这种作用类似于米诺地尔对该动物模型毛发周期动态的作用。通过放射受体测定法测得的C3H小鼠全皮肤中细胞视黄酸结合蛋白的水平在生长期较高，在休止期较低。在毛发周期的两个阶段，局部应用某些类视黄醇都会导致全皮肤匀浆中细胞视黄酸结合蛋白（cRABP）水平升高。在测试的类视黄醇中，那些在改变小鼠皮肤中cRABP水平方面最有效的类视黄醇也能够显著改变毛发周期动态。类视黄醇增加cRABP、增加3H-胸腺嘧啶核苷掺入以及改变毛发周期动态的能力之间似乎存在关联。在人培养的真皮成纤维细胞和真皮乳头细胞中仅可检测到cRABP-II。与真皮乳头细胞相比，真皮成纤维细胞显示出更高量的cRABP-II。cRABP-II表达的差异可能解释了这两种细胞群体对维甲酸的不同反应。cRABP-II表达的差异是否具有生理重要性仍有待确定。用维甲酸处理培养中的人真皮乳头细胞似乎不会影响增殖，至少在所测试的剂量下不会。
