Suda T, Tozawa F, Iwai I, Sato Y, Sumitomo T, Nakano Y, Yamada M, Demura H
Department of Medicine, Tokyo Women's Medical College, Japan.
Brain Res Mol Brain Res. 1993 Jun;18(4):311-5. doi: 10.1016/0169-328x(93)90094-6.
Neuropeptide Y (NPY) has a stimulatory effect on adrenocorticotropin (ACTH) and corticotropin-releasing factor (CRF) release. In the present study, to investigate the effect of NPY on CRF synthesis, the effect of centrally administered NPY on CRF messenger RNA (mRNA) levels in rat hypothalamus was examined under pentobarbital anesthesia. The administration of 0.01, 0.1 and 1 nmol of NPY into the lateral ventricle dose-dependently Increased the plasma ACTH levels, as well as the levels of proopiomelanocortin mRNA in the anterior pituitary. The CRF mRNA level in the hypothalamus also increased after administration of 0.1 and 1 nmol of NPY in a dose-dependent manner. The administration of 3 nmol of phentolamine or propranolol failed to block 0.1 nmol NPY-induced ACTH release or 1 nmol NPY-stimulated CRF mRNA levels in the hypothalamus. These results Indicate that the central administration of NPY increases the CRF mRNA levels in the hypothalamus and the probable CRF release, which increases the proopiomelanocortin mRNA levels and ACTH secretion in the anterior pituitary. Therefore, NPY seems to play a physiological role in the regulation of the release and synthesis of CRF in the hypothalamus.
神经肽Y（NPY）对促肾上腺皮质激素（ACTH）和促肾上腺皮质激素释放因子（CRF）的释放具有刺激作用。在本研究中，为了探究NPY对CRF合成的影响，在戊巴比妥麻醉下检测了向大鼠下丘脑中央给药NPY对CRF信使核糖核酸（mRNA）水平的影响。向侧脑室注射0.01、0.1和1 nmol的NPY可使血浆ACTH水平以及垂体前叶中阿黑皮素原mRNA水平呈剂量依赖性升高。注射0.1和1 nmol的NPY后，下丘脑CRF mRNA水平也呈剂量依赖性升高。注射3 nmol酚妥拉明或普萘洛尔未能阻断0.1 nmol NPY诱导的ACTH释放或1 nmol NPY刺激的下丘脑CRF mRNA水平。这些结果表明，向中央给药NPY可增加下丘脑CRF mRNA水平以及可能的CRF释放，进而增加垂体前叶中阿黑皮素原mRNA水平和ACTH分泌。因此，NPY似乎在下丘脑CRF释放和合成的调节中发挥生理作用。
